The Preliminary Investigation Of Bovine Mastitis Pathogens And Drug Sensitivity Screening In The North China

The production of milk by dairy farmers is not only a business but also a way of life. Many of the decisions are taken on the farm are consequently dictated by both financial constraints and job satisfaction. However, Bovine mastitis remains a major global dairy farming challenge despite the widespread implementation of mastitis control strategies. For bovine dairy mastitis caused by pathogenic bacteria, antibiotics have historically been used to treat the infections. This drug resistance occurs as a result of the frequent use of antibiotic drugs on the animals either through oral administration, parental administration, in food supplements as growth promotants and as intramammary infusions for the treatment of mastitis/ udder infections. In some cases, animals are injected with antibiotics while on feed rations which contain antibiotics on them. Raw dairy cow milk samples were collected from the North region of the Republic of China. The aim of the study was to isolate and identify Bacteria from raw milk samples using both conventional microbiology and molecular methods. The objectives of the study were to isolate different bacteria from raw cow milk samples, determine the identity of isolates using conventional microbiology methods(Gram staining, and catalase test), Evaluate the level of resistance of isolates to commonly used antibiotics, and use specific 16 S rRNA gene analysis. The general farmer knowledge data was achieved using a baseline questionnaire. The questionnaires were distributed in the dairy farms in Jilin, Heilongjiang, and Inner Mongolia. The results of the antimicrobial susceptibility test showed that the isolates were highly susceptible to Amikacin sulfate, 100%, Gentamycin, 87%, Tetracycline 87%. The isolates showed about 27%, 20%, 20% resistance to Kanamycin, Azithromycin, and Colistin B respectively. The highest 40%, of the isolates, were resistant to Ciprofloxacin. The survey responses showed 71% of all respondents of having a university education, 17.8% senior high school and 11.1% in a junior high school. This high educational background results related to the 75.6% employed responders and 22.2% of the responders looking for work. The farmers knowledge of pathogenic bacteria indicates that there is knowledge of the pathogenic microorganisms is which 80% of the respondents were literate and only 22% didn’t know. This also showed that there are dairy employee education programs in place to train the workers on good dairy practices and public health...