Genetic Analysis And Gene Mapping On Yellow-green Leaf Mutant M32 In Rice

Chlorophyll(Chl) is the main component of photosynthetic pigment, and the process of chlorophyll biosynthesis and decomposition is complicated. The mutant of leaf color are suitable materials for the research in chlorophyll biosynthesis synthesis and metabolism of plants. In this paper, M32, which exhibits yellow-green leaf phenotype was identified from Indica variety, QuanfengB. The mutant leaf color express yellow-green in the whole growth stage, even in the late growth period, the yellow- green color doesn’t transfer to green. Comparing with the wild type, the plant height, productive panicle number per plant, panicle length, flag leaf length, flag leaf width, first branch number and seed setting rate in mutant were decreased. In different period, comparing with the wild type, the Chlorophyll a, Chlorophyll b and total Chlorophyll of M32 was decreased, but chlorophyll a/b values were higher than wild type. TEM show that granal stackes in M32 mutant appear less dense, and lack granal membranes compared with those of wild type.Genetic analysis of the mutant suggested that the yellow-green trait of M32 was controlled by one pair of recessive nuclear gene. The F2 population of Nipponbare/M32 was used as the mapping population. The mutant gene was preliminary located on an interval between markers RM282 and RM156 on Chromosome 3, the genetic distances were 7.0cM and 15.8cM, respectively. The mutant gene was finally located in a 316.8kb DNA region between markers RM7323 and RM3297, temporarily designated as ygl219(yellow-green leaf 219). Within this region, 39 open reading frames(ORF) were predicted. Related with the mutant trait, OsDVR was selected to be the most probably the candidate gene for ygl219.Rice yield potential is closely related to panicle length and productive panicle number per plant. In this paper, by using of the single seed descended method, V20B/CPSLO17 RIL populations were constructed to analyze QTL location for the panicle length and productive panicle number per plant in rice. Interval mapping by MapQTL5 based on the high-density genetic linkage map of SALF markers. In this paper, A total of 7 QTLs were detected, 5 of them responsible for panicle length trait were distributed on chromosome 1 and 6 respectively, with the genotype contribution of 6.41%, 22.22%, 6.15% and 13.01%, and the qPL1-1 was a new QTL. 2 QTLs controlling the productive panicle number per plant were located on chromosome 1 and 4 respectively, with the genotype contribution of 13.15% and 8.18%.