| In aquaculture, the marine culture of Vibrio disease is the 1 kinds of very serious harm, a wide range of popular and infectious diseases.Vibrio harveyi、Vibro Splendidus are two kinds of pathogenic bacteria. Vibrio Harveyi、Vibrio Splendidus are gram-negative bacteria,Vibrio Harveyi is one kind may shine, pathogenic bacteria in water distribution range is very wide, we early to Vibrio Harveyi reports not many, but as Vibrio Harveyi cost the aquaculture industry is more and more big, the nearly 10 years our coverage is becoming more and more.Vibrio Harveyi is some Asian countries(India, Thailand, China, etc.) the primary pathogenic bacteria in farmed prawns, and lots of pathogenic bacteria of mariculture fishes;Vibro Splendidus can also cause the Atlantic salmon, trout, turbot and so on many kinds of fish disease, can make the fish tail bleeding, skin ulcers, and ulcers; Vibro Splendidus or trepang rotten skin disease pathogens in the first place.Now we are still rely on the main means of prevention and treatment of disinfectant and antibiotics, these traditional antibiotic drug abuse, it’s easy to make resistant bacteria is also associated with drug residues such as food safety, so we need to find a new microbial antimicrobial agents. Phage as one kind of new type of antimicrobial agents, it has no side effects, germs, it is difficult to cure the resistance and no residue and so on a series of advantages. More and more wide attention by the people. For the breeding process of potential environmental pollution problems, maintaining the health of the aquaculture industry is of great significance to sustainable development.This study from the farms of water samples by phage of Vibrio Harveyi and phage of Vibrio Splendidus study its biological characteristics, for later use phage treatment of infectious diseases caused by bacteria laid a solid foundation, so it is very important theoretical and practical significance.The results indicated:1、Vibrio Harveyi and Vibrio Splendidus as host, using double AGAR plate method from the waste water samples were isolated from the phage, 1 strain after purification using liquid increment method can get the phage titer of 108. Plaque is round, after 12 hours of training,reached vibrio Harvey phage 2 mm in diameter, brilliant vibrio phage 1.5 mm, center, transparent,clear edges. Phage is higher, after increasing the titer in vitro cracking ability is strong, can be used as a follow-up experiment of experiment materials.2、Phage of Vibrio Harveyi and phage of Vibrio Splendidus physiological and biochemical determination, the results showed: the two phage strains are sensitive to chloroform, prove its tail does not contain the lipid material. 2 strains of phage thermal stability, under the condition of50 ℃, also can keep half the titer of 60 ℃ titer basic is zero. pH stability of strong, effective price in PH between 4-11, when the pH in six to eight 2 strains of phage titer best. The phage of Vibrio Harveyi best infection plural is 0.0001; Phage of Vibrio Splendidus was 0.001. Incubation period of phage of Vibrio Harveyi is 40 minutes, outbreak period for 1 hour. The incubation period of phage of Vibrio Splendidus is 30 minutes;outbreak period for 1 hour.3、By electron microscope 2 phages are silk with tail is 20 surface structure, The phage of Vibrio Harveyi total length of 235 nm, Phage of Vibrio Splendidus total length of 315 nm. With10 different kinds of bacteria to verify the specificity of the two kinds of phage to prove the specificity of the 2 kinds of phage are very strong; But they split the fungus is very narrow, The phage of Vibrio Harveyi can only have a cracking effect on individual strains of other Harvey,Phage of Vibrio Splendidus can only have a cracking effect on host.Sum up, separation, purification of the phage strains were has strong ability of crack bacteria,at the same time also has the certain environmental tolerance, for phage as a new microorganism preparation to treat various aquaculture diseases provides a theoretical support. |
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