| Abiotic stress is one of the main factors of the worldwide food crisis, causing at least 50% decrease of the world’s crop production each year. Corn, an important food, feed and industrial raw material is stress-sensitive plants. Its growth period is vulnerable to disturb by drought, high salinity, low temperature, and many other stress factors. It is necessary to explore the plant pathway in abiotic stress and identify plant resistance genes involved in stress regulation under the dilemma of the growing world population together with the lack of available arable lands. MYB, the most ubiquitous transcription factor family in plants is involved in the regulation of plant life and play an important role in the process of plant resistance to abiotic stress. In this study, we cloned a MYB transcription factor from maize F83 and studied initially basic character of Zm MYBR58 gene and its biological function through the bioinformatics analysis, quantitative real-time PCR, resistance analysis of transgenic plants. The main results of this study are as follows:1 Zm MYBR58 gene, coding of protein total including 433 amino acids, containing a 1302 bp full length CDS was cloned from maize F83 by RT-PCR. Theories molecular weight of predicting protein is 46.0966 KD, theoretical isoelectric point is 9.52, theoretical half-life is 30 h. It is the unstable and hydrophilic protein had lots of hydrophilic amino acids than hydrophobic amino acids. Homology results showed that the Zm MYBR58 gene respectively had consistency with Sorghum bicolor, Seteria italic, Triticum aestivum, Oryza sativa and other crops MYB genes.2 Protein analysis showed protein sequence encoded by Zm MYBR58 gene had only one conservative SANT structure formed 3 α-helical structure to help fold into correct protein structure. It belongs to the MYB-related family.3 According to the upper sequence of the Zm MYBR58, we found a series of cis-acting element involved in the light, hormone, drought, low temperature and the cis-acting element related to tissue-specific expression, predicted that Zm MYBR58 gene might be induced by stress factors such as drought, low temperature and hormone, which was involved in plant response to adversity.4 Expression analysis results displayed that Zm MYBR58 gene with tissue-specific was expressed in all tissues of maize, its expression level was significantly higher in corn germ than in other organizations. The expression mode of Zm MYBR58 gene was induced by drought, high salt, ABA, low temperature and high temperature, and its response degree of expression patterns was different under different stress factors, indicating that Zm MYBR58 gene might be involved in the regulation of hormones/stress-signaling pathway in plant resistance mechanism.5 Zm MYBR58 transgenic resistance plants of Arabidopsis thaliana was required by building CPB-Zm MYBR58 recombinant vector, Agrobacterium-mediated transformation of Arabidopsis thaliana, herbicide screening and PCR identification. Resistance of transgenic plants showed a higher drought resistance than wild-type Arabidopsis under drought stress.Above results indicated that Zm MYBR58 gene was involved in the regulation of plant tolerance to abiotic stress. In this study, the structure and function of Zm MYBR58 were analyzed, which laid the basis for further analysis of the gene involved in the regulation of plant stress response and provided an excellent candidate gene for further using genetic engineering techniques to cultivate resistant varieties of maize. |
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