Relationships Between NDF And ADF Content And Thickness Of The Vascular Bundle Lignification Cells For Leaf And Stem Of Alfalfa

As one of the excellent grass, alfalfa is playing an important role in the agricultural structure adjustment and improving the ecological construction. To study the correlation between the content of NDF and ADF and thickness of lignifying cell, the content of NDF and ADF of leaf and stem in different growth stages of Medicago sativa var. Gannong No. 3, Medicago sativa var. Gannong No. 6, Medicago sativa var.Qingshui and M. sativa. L cv. Algonguin were detected, and the thickness of vascular bundle was analyzed and measured by paraffin section of leaf and stem tissue. The results are as follows:1. There were differences of different degrees in the content of ADF and NDF of leaf and stem, which were taken from the four alfalfa varieties. In different growth periods,the ranking of the content of ADF and NDF of leaf and stem of the four tested materials had slightly difference. Overall, the order of the increased speed of the content of ADF and NDF of the 4 tested materials, from fast to low, was: M. sativa. L cv. Algonguin >Medicago sativa var. Gannong No. 6 > Medicago sativa var. Gannong No. 3> Medicago sativa var. Qingshui.2. Aimed to explore the most suitable growth period for cutting and making paraffin sections, the paraffin section of the leaf and stem structure in different periods were observed, compared and described, and the thickness of vascular bundle was detected.The results showed that, during the whole period, the lignifications of the leaf and stem of different materials had increasing trend, but there were differences in the scope and rate, and M. sativa. L cv. Algonguin had the highest lignification degree,Medicago sativa var. Qingshui had the lowest lignification degree. The rate of the lignifications increased sharply in budding phase.3. There were significantly positive correlation between the content of ADF and NDF and the thickness of vascular bundle of leaf and stem. Differences of different degrees existed between the content of ADF and NDF and the thickness of vascular bundle.And there was no consistent regularity between the correlation coefficients of each growth stage.