Effects Of ADM On The Proliferation And The MRNA Expression Of K_ATP In Human Vascular Smooth Muscle Cells Induced By AngII

objective:To investigate the effect of adrenomedullin (ADM) on the proliferation and the mRNA expression of KATP in human mesentery artery smooth muscle cells induced by AngⅡ, and to explore the mechanism of ADM in depressing blood pressure and relaxing blood vessels.Method:Vascular smooth muscle cells(VSMC) were isolated and cultured from mesenteric artery of non-hypertensive patients undergone laparotomy surgery in vitro.The proliferation activity of mesenteric artery smooth muscle cells was determined by MTT assay;the SUR2B/Kir6.1mRNA expression was detected by reverse transcription-polymerase chain reaction(RT-PCR).The cultured VSMC was randomly divided into placebo group;control group:ADM(10-7、10-8、10-9mol/L) and ADM10-7mol/L+Glyburide group; intervention group:AngⅡ10-6mol/L、>AngⅡ<10-6mol/L+ADM(10-7mol/L、10-8mol/L 10-9 mol/L)>、AngⅡ10-6mol/L+ ADM10-7mol/L+Glyburide group. SPSS13.0 package used.OD values of cell activity are showed as the mean and standard deviation (x±s), comparison between groups using t test, multiple comparison were statistically treated by analysis of variance to examine, the level of the P<0.05 was statistically significant. Results: (1)ADM has no significant effect on the vascular smooth muscle cells...