The Study Of Extract Of Chinese Herbs XLS On Uterine Leiomyomas

Hysteromyoma is a commom benign tumor in female genital system. Some patients have obviously clinical symptoms such as change of menstruation, secondary anemia, pain and infertility. Recently, investigators discuss drug conservative treatment which can avoid operation for the disease. The traditional Chinese medicine treatment becomes the investigative direction in recent years for its special mechanisms and less side reactions.XLS is an extractive from a traditional Chinese medicine prescription. We investigated the treatment effect of XLS in hysteromyoma. The results revealled that XLS has the anti-hyperplasia effect on uterine leiomyoma and it regulates sex hormone and hormone receptor, improves microcirculation. Also it has an anti-inflammatory action.Part one The effects of XLS on mouse hysteromyomaAim:To investigate the effects on the mouse model of hysteromyoma of Chinese herbs extract XLS.Method:The model of hysteromyoma was made by pouring diaethylstilbestrol. The mice were then divided into 7 groups:normal group, model group, positive groups (mifepristone,5mg·kg-1, ig, and GZFL,250mg·kg-1, ig) and XLS group (50mg·kg-1, 100mg·kg-1,200mg·kg-1, ig). After the treatment, the wet uterus were weighted and uterus coefficient was calculated. Histomorphology of uterus was also examined.Result:100mg·kg-1 and 200mg·kg-1 of XLS reduced the uterus wet weight and coefficient (P<0.01),50mg·kg-1 of XLS also ameliorated this two indices when compared to model group. The results showed a dose-effect relationship of the drug. Histological observation suggested XLS declined the thickness of uterus smooth muscle, inhibited the proliferation of smooth muscle cell and improved the histomorphology of hysteromyoma.Conclusion:XLS inhibits the hyperplasia of mouse uterine leiomyoma. Part two The effects of XLS on rat hysteromyomaAim:To investigate the effects of XLS on rat hysteromyoma, estrogen, progestogen and their receptors.Method:The model of hysteromyoma was made by pouring diaethylstilbestrol. Then rats were divided into 7 groups:normal group, model group, positive groups (mifepristone,5mg·kg-1, ig and GZFL,250mg·kg-1, ig) and XLS group (25mg·kg-1, 50mg·kg-1, 100mg·kg-1, ig). After treatment, the level of estradiol and progesterone in rats’blood serum were detected by using chemoluminescence method. Estrogen receptor and progesterone receptor of uterus smooth muscle cell were detected by immune histochemical staining.Result:The level of estradiol was decreased sharply in all treatment groups (P<0.01), when compared to model group. The high dose of XLS and mifepristone had the best effect. Histological observation found that the receptor in normal rat uterus smooth muscle were weak and scattered, the receptor density in model group was increased, and had statistical significance compared to normal group (P<0.01). After treatment by XLS and mifepristone, the receptor density was declined, compared to model group (P<0.01).Conclusion:XLS can decrease the level of estradiol and inhibit the expression of estrogen receptor and progesterone receptor in uterus smooth muscle cells.Part three The effects of XLS on microcirculationAim:To investigate the effects of XLS on mouse auricle microcirculationMethod:Mice were devided into 5 groups:normal group, positive group (Danshen Injection,2mL, ig), XLS group(50mg·kg-1, 100mg·kg-1,200mg·kg-1, ig). After anaesthesia, the microcirculation of mouse auricle was examined. The microcirculation was observed before administration and at 5min, 10min,15min, 20min,25min and 30min after drug. We use Image-Pro Plus to analyze the microvascular diameter and blood flow velocity.Result:After mice were treated with XLS and Danshen Injection, the microvascular diameter was larger at different time points than before administration, and also had statistical significance comparing to normal group (P<0.01). In part of the time points, the microvascular blood flow rate in all treatment groups was significantly faster than that before administration, and was also faster than normal control group at the same times.Conclsion:XLS can dilate microvessels, increase microvascular blood flow velocity and finally improve the microcirculation.Part four The effects of XLS on inflammationAim:To observe the effects of XLS on mouse ear swelling and rat foot swelling.Method:Mice were randomly divided into 5 groups:normal group, positive group (GZFL,650 mg·kg-1, ig), XLS group (70mg·kg-1,140mg·kg-1,280mg·kg-1, ig).We used dimethylbenzene to induce ear inflammation, tested the degree of mice ear swelling and the inhibition rate of swelling. In another protocol, rats were also randomly divided into 5 groups:normal group, positive group (GZFL,460 mg·kg-1, ig), XLS group (50mg·kg-1, 100mg·kg-1,200mg·kg-1, ig).We used egg white to induce the inflammation, tested the degree of rat foot swelling and the inhibition rate.Result:Swelling of mouse ears induced by dimethylbenzene was significantly inhibited in all treated groups and dose-effect relationship was shown. Giving treatment decreased the degree of swelling, and different dose of XLS appeared a different time-effect relationship. 100mg·kg-1,200mg·kg-1 XLS and GZFL had the maximum inhibition rate of rat foot swelling at 1.5h after inflammation, while 50mg·kg-1 XLS was in 2h.Conclsion:XLS can inhibit the swelling of mouse ear and rat foot in inflammation, indicating that it has anti-inflammatory action.Part five The acute toxicity of XLSAim:To observe the acute toxicity of XLS in mouse.Method:With the single maximum dose of 6000 mg·kg-1, XLS was administered into mice, and any change of mice was monitored for 14 days after administration.Result:In two weeks, none of the mice was dead, the mice were quite normal and body weight was increased. On the 15th day mice were sacrificed, no abnormality observed in autopsy.Conclsion:XLS is a hypotoxicity drug.