The Changes Of Serum Testosterone And Cortisol In Injury Of Heart Of Exhaustive Exercised Rats And Effects Of Chinese Herbal Drugs

Exhaustive exercise often leads to heart injury in training of athletes or soldiers. Itsmechanism is not yet fully clear. Chinese medicine believes that long period of excessiveexercise will induce consume energy, resulting or deficiency of heart-energy.ABSTRACT AIM：To study the changes of serum testosterone and cortisol in heart injuryof exhaustive rats as well as the effects of chinese herbal drug“-Xinfukang Oral Solution” onthem, therefore provide information for prevention and treatment of heart injury.METHODS：40male Sprague-Dawley rats were randomly divided into quiet control (C)group (n=10), one-time exhaustive exercise (E) group (n=10), Xinfukang Oral Solution (L)group of low dose and Xinfukang Oral Solution (H) group of high dose. Rats were killed aftersingle bout of exhaustive swimming. Group C and Group E were gavaged with distilled waterfor10days. Group C weren’t treated. Group E underwent one-time exhaustive swimming.Group L and Group H were gavaged with XinfukangOral Solution for10days, then bothgroups underwent one-time exhaustive swimming. Each group was narcotized with10%hydral and serum specimen was taken to measure the activity of MB isoenzyme of creatinekinase (CK-MB) and levels of cardiac troponin I （cTnI）, testosterone and cortisol.Myocardium samples were taken to observe histomorphologic changes by light microscopeand electron microscope afterwards.RESULTS：1. Histomorphology of myocardium samples observed by light and electron microscopy:cardiac sarcomeres of Group C rat were arranged in neat rows, the density was uniform andthere was no organelle edema, mitochondrial membrane and ridge were normal; Theperinuclear matrix of myocardium of Group E suffered a high degree of edema and widerperinuclear gap. the number of mitochondria and glycogen reduced significantly. Part of theridge and part of membrane mitochondria of perinuclear matrix fused, blurred or missed, asmall amount of myocardium underwent fiber necrosis. In Group L and H, part of the ridgeand part of membrane mitochondria of perinuclear matrix fused, blurred or missed. 2. The result of the swimming time: The average swimming time of Group E issignificantly lower than that of Group L and Group H（363.30±60.78:420.80±34.66and483.20±43.37min, P＜0.01）. The average swimming time of Group L is significantly lowerthan Group H（420.80±34.66:483.20±43.37min, P＜0.01）.3. Serum cTnI assay results: The level of serum cTnI of Group E is significantly higherthan Group C, Group L and Group H （1.4077±1.3086：0.5141±0.3677,0.5002±0.2580and0.4504±0.3288ng/mL, P＜0.05）; That of Group C is higher than Group L and Group H,yet there is no significant difference（0.5141±0.3677:0.5002±0.2580and0.4504±0.3288ng/mL, P>0.05); The level of serum cTnI of Group L is higher than Group H, there is nosignificant difference either（0.5002±0.2580:0.4504±0.3288ng/mL, P>0.05).4. Serum CK-MB assay results: Serum CK-MB activity of Group C is significantlylower than Group E, Group L and Group H （568.70±107.09:1547.80±393.62,1030.10±370.19and1146.00±260.00U/L, P＜0.01）. Serum CK-MB activity of Group E issignificantly higher than Group L and Group H（1547.80±393.62:1030.10±370.19U/L，P＜0.01;1547.80±393.62:1146.00±260.00U/L, P＜0.05）. Serum CK-MB activity of GroupL is significantly lower than Group H（1030.10±370.19:1146.00±260.00U/L, P＞0.05）.5. Serum testosterone assay results: Serum testosterone level of Group C is significantlyhigher than Group E, Group L and Group H （2.73±0.51:0.82±0.11,1.24±0.12and1.30±0.20ng/mL, P＜0.01）. Serum testosterone level of Group E is significantly lower thanGroup L and Group H（0.82±0.11:1.24±0.12ng/mL, P＜0.05;0.82±0.11:1.30±0.20ng/mL, P＜0.01). Serum testosterone level of Group L is significantly lower than Group H（1.24±0.12:1.30±0.20ng/mL, P＞0.05）.6. Serum cortisol assay results: Serum cortisol level of Group C is significantly lowerthan Group E and Group L （13.15±3.00:22.13±2.98, P＜0.01;13.15±3.00:17.62±2.34ng/mL, P＜0.05）, Serum cortisol level of Group E is significantly higher than Group L（22.13±2.98:17.62±2.34, P＜0.05）. Serum cortisol level of Group H is higher than Group C,Group E and Group L（28.08±7.71:13.15±3.00,22.13±2.98and17.62±2.34ng/mL, P＜0.01）. Conclusion：Exhaustive exercise has a significant injury to rat myocardium. Theincrease in serum cortisol levels and the decrease of testosterone may have been involved inmyocardial injury. XinfukangOral Solution has significant effect on protecting exhausiveheart injury of rats, and can increase exercise tolerance in rats significantly. These effectspossible work through lowering cortisol and increasing testosterone therefore protect heart.However, the protective effect showed no dose-dependent tendency. Exercise toleranceincreases with apparent dose-dependent tendency. This research may provide reference forprevention and treatment of myocardial injury induced by exhaustive excercise in athletes andmilitary men.