Short-term Ischaemic Storage Of Human Uterine-basic Studies Towards Uterine Transplantation

BackgroudUterine factor infertility is either due to congenital malformation or acquired.Uterus transplantation may be a possible treatment option in the future for absoluteuterine infertility. For uterines transplantation, graft operation technical problemsshould to be solved associated with rejection, immunosuppression, graft uterinepregnancy, parturition and fetal growth and development related issues. While in thetransplant operation, The tolerance of human uterine tissue to cold ischemicpreservation is one of the issues that need to be resolved.Cold storage with aprotective buffer is today the most common method used to preserve an organintended for transplantation during the ischemic time between procurement andreperfusion within the recipet.Lowering of tissue metabolism by hypothermia togetherwith the protective properties of a transplant solution slows down the development ofischemic injuries, Ischaemia causes a rapid decline in ATP concentrations resulting inan inability of cells to maintain homeostasis.so the tolerabe time window is stillrestricted.For example,the clinically practiced time limit for cold preservation rangesfrom6hours for hearts up to36hours for kidnevs. The aim of the current study wasto explore The tolerability of human uterine tissue to cold ischaemic storage and tocompare the effect of different preservation solutions.then the finding can provide corresponding guidance for the future of the uterine transplantation or organ cloningtechnology in uterine preservation.PurposeSmall tissue samples of human uteri were subjected to cold (4℃) ischaemia (6and24h) in Rs solution,the UW solution and the HTK solution.Some importantmeans were used as parameters to detect cold ischaemic injuries.Then we can explorethe tolerability of human uterine tissue to cold ischaemic storage and to compare theeffect of different preservation solutions,and we hoped that the finding can providecorresponding guidance for the future of the uterine transplantation or organ cloningtechnology in uterine preservation.Explore The tolerability of human uterine tissue to cold ischaemicstorage.MethodsSmall tissue samples of human uteri were subjected to cold (4℃) ischaemia (6and24h) in Rs solution and the UW solution.some means as blow was used to assessevidence of cold ischemic injury:1. Histological analysis by light microscopy.2. Histological analysis by electron microscopy.3. The ability of myometrial strips to contract.ResultOn evaluation by light microscopy, no apparent histological changes were seenin the myometrium or the endometrium after cold ischaemic preservation in any of thesolutions for6or24h.there was no evidence of cellular swelling or degenerativechanges of the longitudinal muscle striations among the different preserved uterinetissue samples.Using electron microscopy, degenerative changes such as hydropic degenerationand slight nuclear changes (coarse chromatin) were observed in tissue stored for24h in RIN. Tissue stored for6h in any of the solutions and for24h in UW displayed nomorphological changes compared with the control.Cold storage in any of the solutions decreased the ability of the myometrialspecimens to start contractions spontaneously in comparison with control. allspecimens stored in UW and RS for6h contracted either spontaneously or afterstimulation with a low concentration of oxytocin.However, those stored in RIN for24h where no one specimen produced contractions. Specimens stored in UW for6hproduced curves and responded to oxytocin in a similar manner to controls, whileafter24h storage the quality of the contractions and responses were clearly altered.The RS-preserved specimens showed a contraction pattern that was less regular andrhythmic compared with control and specimens stored in UW.conclusionhuman uterine myometrial tissue is resistant towards cold ischaemia for at least6h,and the human uterine myometrial tissue is resistant towards cold ischaemia in UWpreservation solution for up to24hours.Compare the effect of the UW solution and the HTK solutionMethodsSmall tissue samples of human uteri were subjected to cold (4℃) ischaemia (6and24h) in RS solution,the UW solution and the HTK solution. some means as blow wasused to compare the efficacy of the UW solution and the HTK solution:1. the levels of the Na+-K+ATPase activity2.The contents of GST and GSH.ResultAt each time point.there was no significant difference in the activity of Na+-K+ATPasebetween UW group and HTK group(P>0.05), Compared with RS solution．the levelsof the Na~+-K~+ATPase activity preserved with UW solution and HTK solution werehigher(P<0.05).At6h. there was no significant difference in the contents of GSH between UW group and HTK group(P>0.05).but at24h, The GSH was significantly higher in specimenspreserved in UW group compared with HTK group(P<0.05). Compared with RSsolution．the levels of the contents of GSH preserved with UW solution and HTKsolution were higher(P<0.05).ConclusionHTK solution has a similar preservative effect on short-term uterine storage to that ofUW solution. The effect of UW solution may be better than that of HTK solution onlong-term storage.