Study Of Neurobehavior And Different Proteins In Hippocampal Of Rats Induced By Sub-chronically Benzo[a]pyrene

Objective:1. To observe the behaviour and mental status of sub-chronically benzo[a]pyrene rat,study theinfluene to the behaviour and mental caused by benzo[a]pyrene.2. Study the different proteins of the groups, discuss the nerve damage mechanism ofbenzo[a]pyrene.Methods50healthy adult male SD rats were randomly divided into5groups: blank control group,olive control group, B[a]P infected group, GGA+oliver control group and GGA+B[a]P infectedgroup, except the blank control group, the other4groups were exposured to oliver or B[a]p byintroperitioneal on alternate day, the next day GGA+oliver control group and GGA+B[a]Pinfected group were exposure to GGA by gavage administration. thewhich were administeredintra-peritoncally for90days, the exposure lasting90days. The Morris water maze test wasperfomed to test the learning and memory abilities of rat. Separate the different proteins by2-DE,analysis the proteins which differently expressed in different groups, then determine the proteinsby mass-spectrometric technique.ResultsAfter the end of the exposure, the body weight was significantly lower than that of the blankgroups, the solvent group, the difference was statistically significant (p0.05).The results of Morris water maze test show, the escape latencies of navigation test and theaverage escape latencies of navigation test of the exposure group were significantly higher thanthat of the blank control group, olive control group, GGA+oliver control group and GGA+B[a]Pinfected group, the difference was statistically significant (p0.05).The escape latencies ofnavigation test and the average escape latencies of navigation test of GGA+B[a]P infected groupwere significantly higher than that of blank group, the difference was statistically significant(p0.05). Durations of the third quadrant of the B[a]P infected group were significantly lowerthan that of the other4groups, the difference was statistically significant (p0.05). The total roadof navigation test of the B[a]P infected group were significantly higher than that of the the blankcontrol group, olive control group, GGA+oliver control group, the difference was statisticallysignificant (p0.05).The result of2-DE: GGA+olive group have9spots more than olive control group,15spots up-regulate,18spots down-regulate, olive control group have5spots more than GGA+olivecontrol group; B[a]P infected group have5spots more than olive control group,14spotsup-regulate,13spots down-regulate, olive control group have5spots more than B[a]P infectedgroup; B[a]P infected group have6spots more than GGA+B[a]P infected group,17spotsup-regulate,18spots down-regulate, GGA+B[a]P infected group have6spots more than B[a]Pinfected group; GGA+B[a]P infected group have7spots more than GGA+olive control group,24spots up-regulate,10spots down-regulate, GGA+olive control group have7spots more thanGGA+B[a]P infected group.47distinct protein was identified by Mass Spectroscope, affirm53proteins, including23energy metabolism proteins,12skeleton proteins,4Neuromodulation related proteins,4unknown proteins,3signal transduction proteins,3Tumor-associated membrane proteins,2Oxidative Stress proteins and2Molecular chaperone.Conclusions1Sub-chronic exposed to B[a]P impair learning and memory ability of rats.2GGA can protect rat from impairing learning and memory ability.3Ayalysing the difference protein in hippocampus, containing energy metabolism protein,skeleton protein, neuroregulation correlated protein, signal transduction protein, oxidative stressprotein, molecular chaperone protein. Some of those proteins may correlate to the function andmetabolism of neuron system, and provide thought and proof to the mechanism of B[a]P’sneurotoxicity.