Bioreactor Application To Establish Production Technology Of Hemorrhagic Fever With Renal Syndrome Bivalent Vaccine(Vero Cell), Inactivated

Hemorrhagic fever with renal syndrome is a kind of naturalimmune infectious disease, is a rodent carrying and spreadingcaused by fever, bleeding tendency and kidney damage as the mainclinical feature of acute infectious diseases. This diseasethroughout all continents of the world, they are mainly distributedin eurasia,. In recent years the world has more than150000casesof hemorrhagic fever cases, death of thousands of cases, China’sannual report the incidence of2-3million people, up to100000, isthe world’s fever number90%, has become an addition to viralhepatitis, the greatest harm a viral disease. In addition to therodent prevention measures for the control and prevention of thedisease, the use of safe and effective vaccines is to reduce theincidence of epidemic outbreaks, control key. The current marketcirculation of vaccines against hemorrhagic fever with renalsyndrome is a primary cell and Vero cell matrix for preparation ofthe bivalent vaccine, with primary cells to matrix preparationvaccine requires a large amount of animal, is easy to pollute theexogenous factor; and to Vero cell based vaccine preparation in recent years, branch of national medicine inspect their qualityrequirements more and more high, especially the host DNA residuesand host protein content control is many enterprises are difficultto prepare qualified vaccine, analysis its reason mainly is thecurrent production process are used to bottle preparation virusliquid, low virulence of the virus harvest liquid high concentrated,with raising concentration multiple host DNA and host proteinresidues also increased, so it is difficult to make high qualityof vaccine, so the virus virulence is harvested liquid vaccinequality is a key factor, to obtain high virulence of the virusharvest fluid only by biological reactor for high cell densitycultivation by infection, high cell density to obtain highvirulence of the virus harvest fluid.This experiment by biological reactor for preparing highvirulence of the virus liquid, low multiple concentration in liquidafter virus inactivation of purified liquid obtained after price,certified by the unit liquid with1:1ratio in the mixture by addingthe right amount of virus stabilizer and aluminum hydroxideadjuvant preparation of hemorrhagic fever vaccine. Theexperimental data shows that the process is suitable for industrialproduction and can reduce the cost, increase yield.