Production And Purification Interferon α-2b Protein

Interferons (IFNs) are produced and released in response to the infection ofpathogens or tumor cells.Interferon belongs to a kind of glycoprotein and has abilityof its interference with virus replication in the host cell. Interferon can increase theidentification of the immune system on pathogens and resist virus infection.Nowinterferon is widely used in the treatment of viral diseases and tumors. Interferons(IFNs) are produced and released in response to the infection of pathogens or tumorcells. Interferon with antiviral, anti-tumor, regulate the immune system functions.Interferon has been widely used in the treatment of viral diseases and cancer,However, to improve the production of interferon protein is the most critical part.With the development of recombinant DNA technology. It has been using the E.coliexpression system for expression of interferon genes. Interferon protein in the form ofinclusion bodies, so you need to denaturation and refolding of inclusion body proteinsto get a natural interferon protein, however, this step can easily aggregates andmisfolded, this problem has been the most critical part of interferon production, Thispaper introduces the method of inclusion body protein refolding to discuss and resolvethe problem of improving the production of interferon protein.Improve ion exchange chromatography separation of interferon α-2b dimers Bycomparing the separation of glycerol and without glycerol group to get the bestseparation method. The effect of the mobile phase of25%glycerol can effectivelyimprove the ion exchange chromatographic separation of interferon α-2b dimer.