DHA Supplementation During Pregnancy On Intrauterine Infection Cause Fetal Rats Brain Tissue The Expression Of S-100B, ROS And IL-1β

Objectives:To research the expressions of S-100B protein (S-100B), reactive oxygenspecies (ROS) and interleukin-1β (IL-1β) in brain tissue of fetal rats by preparingthe animal models of brain injury caused by infection in utero and adding DHAduring pregnancy.Then research the role of S-100B protein (S-100B), reactive oxygenspecies (ROS) and interleukin-1β (IL-1β) in white matter damage,as well as theprotective effects of DHA after intrauterine infection in brain tissue of fetal rats.Method:A total of30female Sprague-Dawley(SD) rats that determined the first day ofpregnancy were randomly divided into DHA intervention group (DHA group), LPSgroup (LPS group) and normal saline group (NS group). Each group has10rats.Each of pregnant rats of DHA group were given DHA130mg per day from the firstday of pregnancy to childbirth. Pregnant rats of LPS group and NS group were givenequal volume of sterile saline at the same time. During the period of the17th and18thdays of gestation,the pregnant rats of the DHA group and the LPS group wereinjected LPS(350ug/kg) in enterocoelia, while the NS group were injected with thesame volume (0.6ml) of sterile saline.We took the pups brain tissue of each groupwhen in the pregnant20days, the1,3,7days after birth (denoted as G20, P1, P3, P7),and placenta of pregnant rats in each group during childbirth.We observe pathologicalchanges about the placenta of pregnant rats and the pups brain tissue of each group atdifferent time points in the light microscop after using hematoxylin-eosin staining(HEstaining). We applicated SP immunohistochemical method to detect the expression ofS-100B and IL-1β at different time points in pups brain tissue,and enzyme-linkedimmunosorbent assay kit (ELISA kit) to detect the level of ROS. Results:1.The HE staining about pregnant rats,uterus and placenta:In LPS group, uterineand placental tissue were congestion and edema significantly,and seen a lot ofneutrophils.In NS group,uterine and placental tissue were arranged in neat rows,weren’t congestion and edema,and inflammation was not observed apparently.Whilethe DHA group were between the LPS group and the NS group.2.The HE staining about the fetal rats’brain tissue:In LPS group, the nerve cellswere swelling,even with focal hemorrhage.The white matter nerve fibers wereirregular and the number of cells were reduced in the after period.In DHA group,nerve cells were edema mild in the early time, but couldn’t be seen obvious focalhemorrhage.The white matter nerve fibers were slight irregular and the number ofcells were normal in the after period.The brain tissue in NS group were normalalways.3.The expression of S-100B in brain tissue:In LPS group,the expression ofS-100B reached summit at the G20,then decreased gradually at P1,P3,P7.Whencompared with the NS group,the difference was statistically significant at the G20, P1,P3, P7(P <0.01). In DHA group,the expression of S-100B compared with thecorresponding time points in LPS group were decreased significantly.The differencebetween them was statistically significant (P <0.01).When the DHA group comparedwith the NS group, the difference was statistically significant in the G20, P1, P3(P<0.01), but without statistically significant in P7(P>0.05).4.The expression of ROS in brain tissue:In LPS group,the expression of ROSreached summit at the G20,then decreased gradually at P1,P3,P7.When comparedwith the NS group,the difference was statistically significant at the G20, P1, P3, P7(P <0.01). In DHA group,the expression of ROS compared with the correspondingtime points in LPS group were decreased significantly.The difference between themwas statistically significant (P <0.01).When the DHA group compared with the NSgroup, the difference was statistically significant in the G20, P1, P3(P <0.01), butwithout statistically significant in P7(P>0.05).5.The expression of IL-1β in brain tissue:In LPS group,the expression of IL-1β reached summit at the G20,then decreased gradually at P1,P3,P7.When comparedwith the NS group,the difference was statistically significant at the G20, P1, P3, P7(P <0.01). In DHA group,the expression of IL-1β compared with the correspondingtime points in LPS group were decreased significantly.The difference between themwas statistically significant (P <0.01).When the DHA group compared with the NSgroup, the difference was statistically significant in the G20, P1, P3(P <0.01), butwithout statistically significant in P7(P>0.05).Conclusion:1.We can see a lot of inflammatory cells, irregular white matter fibers and lightlystained nucleus in the brain tissue after intraperitoneal injection of LPS by lightmicroscopy. The effect of the test indicat that intrauterine infection can lead tooffspring of white matter damage.2.Compared with the NS group,the expression of S-100B, ROS and IL-1β in thebrain tissue among the LPS group were increased significantly. The difference wasstatistically significant (P <0.01). The effect of the test indicat that a large number ofinflammatory cytokines and inflammation-related releasing factors after intrauterineinfection,which may play an important role in white matter injury.3.Compared with the LPS group, the pathological changes of brain were redusedas well as the expression of S-100B,ROS and IL-1βin the DHA group. The effect ofthe test indicat that DHA may plays a certain protective effects of inflammation,anti-oxidation in the white matter damage caused by intrauterine infection.4.There are significant positive correlation between the expression of S-100BROS and IL-1β in the fetal rats’ brain tissue after intrauterine infection. The effect ofthe test indicat that exist a signaling pathway mediating the brain damage betweenthem possibly.