Objective: To investigate whether Orai1or STIM1can cause the pathogenesis ofpulmonary hypertension by regulating calcium inside flow and pulmonary arterysmooth muscle cell proliferation or not.Method: Separate PASMCs of normal rats in the sterile environment, and culture invitro. Establish STIM1and Orai1eukaryotic expression vector. Let Orai1and STIM1expression vector transfects normal PASMCs respectively, extract RNA and proteinsof those PASMCs48-72h later, and use real time PCR, Western blot to detecte theexpression of those genes. Detect the effects of those genes on PASMCs proliferationby cell counting kit-8.Results: Pulmonary arterial smooth muscle cells were separated and cultured. STIM1and Orai1genes were transfected into PASMCs. The increased mRNA and proteinlevels of these genes were confirmed by RT-PCR and Western-blot, respectively.Overexpression of STIM1or Orai1increased the proliferation efficiencies ofPASMCs.Conclusion: STIM1and Orai1genes over-expressions increased pulmonary arterysmooth muscle cells proliferation. |