Research On Surface Morphology And Mechanical Properties Of Cervical Cancer Cell

Tumor divided into benign and malignant tumor, malignant tumor is theso-called cancer. Cancer has particularly high mortality rate among diseases, andthe number of cancer patients is increasing every year. One of the importantreasons for high malignant tumor mortality is that the early diagnosis isdifficult.Most diagnosed is already terminal cancer, patients missed the besttreatment time resulting in death. Human studies on the pathogenesis andmetastasis of cancer invasion mechanism have no fundamental understanding. Inrecent years, people studied from the angle of mechanics properties about cancer.Because cell is the basic structure and functional unit of an organism, somechanical characterization of single-cell level has great significance.Through a large number of studies,people have found that once normal cellsoccur lesions,its structure and function would changes and also mechanicalproperties. The mechanical properties is different from the lesion severity.With thedevelopment of nanotechnology, a variety of cell mechanical propertiesexperimental technology is becoming more and more mature. Atomic forcemicroscope is a very effective tool among biomedical fields. It has the advantagesof high resolution and high sensitivity of nanometer level.Cell surface informationacquisition and mechanical properties characterization is very convenient. Thistopic is mainly study on surface morphology and mechanical properties of cancercells based on AFM.Using atomic force microscope conduct scanning experiment to observe themicrostructure on the surface of the cancer cells.Cells were scanned in contactmode. Select the appropriate parameters to ensure that the topography imagingquality is good.Collect cancer cells’ surface microtopography andthree-dimensional topography in different channel mode, analyze themicro-structure and observe the three-dimensional topography of the cell,determine the general size and thickness of the cell and cell surface roughnessvalues.In addition, surface morphology was studied by using laser confocalmicroscope and scanning electron microscope.Established probe cells creasing model by using the finite element analysissoftware ABAQUS, using the parameters obtained from the experiment, get thecell stress and deformation, load displacement curves and compared with thetheoretical value. Change parameters such as cell thickness,coefficient offriction,ball head radius,poisson’s ratio and so on,then analysis the effect on the load-displacement curve.Using atomic force microscopy indentation experiment was conducted tocharacterize the cancer cell mechanical properties.Indentation experiment get theload-displacement curve, then use the Hertz model fitting for the elastic modulusof cancer cells.The indentation of different areas would get the different elasticmodulus value.Through the analysis of the indentation tip geometry andmathematical model selection discuss the influence on cell mechanics properties.