Study On The Biological Mechanisms In Adjustment Premature Ovarian Failure About Ding Jing Tang And Its Separations Based On Factor Analysis

Objective: Composite mental stimulation was used to establish a rat model ofpremature ovarian failure. Rat was administrated to observe the pharmacodynamicabout Ding Jing Tang and its separations in Adjustment premature ovarian failure.Internal analyzed rat reproductive endocrine indicators by factor analysis to explorethe adjustment mechanism in premature ovarian failure.Methods：sound-light-electric multiple stress stimulation was used to copy therat model in premature ovarian failure. Rats were divided into7groups: normal group,model group, whole decoction group, main drugs group, no major drugs group,shugan group and bushen group. All rats were made model by multiple stressstimulation from the first day except normal group. Normal group and model groupwere given normal saline. Anther groups were given by corresponding drug. After20days, E2, P, FSH, LH, ACTH, CORT and NO were detected in Serum, GnRH, β-EP,IL-1, CRH, NOS, ER and PR were detected in hypothalamic homogenate at rat estrusto observe pharmacodynamic. Regulation relations of biological molecules wereexplored in each group based on factor analysis. And to explore the key biomoleculesin premature ovarian failure which caused by psychological stress.Results: Observing the changes of rat signs, estrous cycle, visceral weight andmorphology. It was obvious difference between normal and model group. There werestatistical differences between two groups by detecting biochemical indicators (E2, P,FSH, LH, ACTH, CORT, GnRH, β-EP, IL-1, CRH) which relate to premature ovarianfailure (P<0.01or P<0.05).However, compared with the POF model group, intervened by giving herbal compound like whole decoction group was significantly improved E2, LH, NO, GnRH,NOS, ER and PR level (P<0.01or P<0.05), and reduced FSH, ACTH, CORT, β-EPand IL-1level (P<0.01). Main drugs group was significantly improved E2, GnRH,NOS, ER and PR level (P<0.01or P<0.05), and reduced FSH, ACTH, CORT andβ-EP level (P<0.01or P<0.05). No major drugs group was significantly improved E2,P, LH, NO, GnRH, NOS, ER and PR level (P<0.01or P<0.05), and reduced FSH,ACTH, CORT, β-EP, IL-1and CRH level (P<0.01or P<0.05). Shugan group wassignificantly improved E2, P, GnRH, NOS, ER and PR level (P<0.01or P<0.05), andreduced FSH, ACTH, CORT, β-EP and CRH level (P<0.01or P<0.05). Bushen groupwas significantly improved E2, P, LH, NO, GnRH, NOS, ER and PR level (P<0.01orP<0.05), and reduced FSH, ACTH, CORT and CRH level (P<0.01or P<0.05).1have strong contact with β-EP, IL-1, CRH, E2, FSH, PR and GnRH, whilesome contact with P and ER, and the variance contribution rate of39.899%in normalstate.2have strong contact with NOS, ER, CORT and NO, while some contact withE2, FSH and GnRH, and the variance contribution rate of20.815%.3have strongcontact with ACTH and LH, while have some contact with NO and P, and thevariance contribution rate of11.490%.1have strong contact with FSH, LH, ACTH, E2, P, PR, CORT and NOS, whilesome contact with P and ER, and the variance contribution rate of46.043%inpsychological stress state.2have strong contact with GnRH, CRH, β-EP and IL-1,while some contact with E2, FSH and NOS, and the variance contribution rate of14.255%.3have strong contact with ER, while have some contact with CORT, andthe variance contribution rate of10.546%.4have strong contact with NO, and thevariance contribution rate of7.834%.1have strong contact with P, E2, NO, FSH, LH, PR and NOS, while somecontact with IL-1and CORT, and the variance contribution rate of44.551%in givenby whole decoction group intervention state.2have strong contact with β-EP,GnRH, IL-1and CRH, and the variance contribution rate of19.512%.3have strongcontact with ACTH and CORT, while have some contact with FSH, NOS and ER, and the variance contribution rate of8.622%.1have strong contact with LH, E2, FSH, P, PR and NOS, while some contactwith CORT, GnRH and ACTH, and the variance contribution rate of37.770%ingiven by main drugs intervention state.2have strong contact with β-EP, IL-1andCRH, and the variance contribution rate of18.046%.3have strong contact with PR,while have some contact with FSH, ER and CORT, and the variance contribution rateof12.339%.4have strong contact with NO and GnRH, while have some contactwith CORT and ACTH, and the variance contribution rate of6.916%.1have strong contact with FSH, E2, P, LH, PR and NOS, while some contactwith NO, β-EP and IL-1, and the variance contribution rate of45.256%in given by nomajor drugs intervention state.2have strong contact with GnRH, CRH, β-EP, IL-1and ER, while some contact with NOS, and the variance contribution rate of22.810%.3have strong contact with ACTH and CORT, while have some contact with PR andNOS, and the variance contribution rate of8.884%.1have strong contact with LH, E2, FSH, P and NO, while some contact withβ-EP, CRH, IL-1, NOS and PR, and the variance contribution rate of47.811%ingiven by shugan drugs intervention state.2have strong contact with GnRH, β-EP,CRH and IL-1, and the variance contribution rate of14.020%.3have strong contactwith ACTH, NOS and CORT, while have some contact with FSH, and the variancecontribution rate of9.528%.4have strong contact with ER and PR, and thevariance contribution rate of7.562%.1have strong contact with NOS, E2, FSH, LH, P and PR, while some contactwith CORT, GnRH, IL-1and CRH, and the variance contribution rate of42.912%ingiven by bushen drugs intervention state.2have strong contact with GnRH, β-EP,CRH and IL-1, while some contact with FSH, and the variance contribution rate of16.153%.3have strong contact with NO, while have some contact with ER, and thevariance contribution rate of9.634%.4have strong contact with ACTH, while havesome contact with ER, and the variance contribution rate of7.457%. Conclusions: It was feasible that the rat models of premature ovarian failurewere set up by composite mental stimulation, and molded successfully. Ding JingTang and its separations can effectively improve E2, P, LH, NO, GnRH, NOS, ER andPR levels, and reduce FSH, ACTH, CORT, β-EP, IL-1and CRH levels. Theregulation of hypothalamus biological molecules to HPO axis plays a dominant role innormal state. Dominant role was that the activated HPA axis biomolecules regulationto HPO axis and hypothalamus in psychological stress state. While the major role wasHPO axis biomolecules regulation of the hypothalamus and the HPA axis in theintervention state of whole decoction group, main drugs group and bushen drugsgroup. HPO axis biomolecules plays a dominant role in the regulation to thehypothalamus in given by no major drugs intervention state. As well as shugan group.