| In recent years, as people sure of oral implantology, more and more patients with lossof teeth choose implantation technology. And titanium implants are naturally widespreadused due to their good biocompatibility and biomechanical properties in the field of oralmedicine. Despite follow-up studies have indicated that10-year of success rate abouttitanium implants has achieved90%-95%, the cases with failed implants still happened.Studies showed that bacterial biofilm on the implant surface is the main cause of earlypostoperative infection, and may lead eventually to implant treatment failure. The currentresearch mainly focused on constructing antibacterial coatings on titanium implantssurface, which can inhibit early bacterial adhesion. Halogenated furanone compounds areone of the newly discovered antimicrobial in recent years, and have been demonstrated tohave many advantages-widely antibacterial spectrum and can effectively restrain thegrowth of the bacterial biofilm, not easy to produce bacterial drug resistance, has good biological compatibility, no genetic toxicity and cell toxicity, etc. In view of this, ourresearch team has fabricated a novel antibacterial coating containing BBF-loadedpoly(L-lactic acid)(PLLA) nanoparticles on microarc-oxidized titanium. The projectpreliminary studies indicated that the antibacterial coating have lasting (as long as60days)and efficient antibacterial performance in addition to good osteoblast compatibility. Buttheir practical effect in the complex oral environment is not clear.Therefore, implants, which containing antimicrobial coating-halogenated furanone,will be implanted in the mandible of Beagle dogs. Then induce infection, which occursaround implants, through silk ligation and feeding high sugar diet. Implants treated withMinocycline Hydrochloride Ointment (once per week) to be used as positive control groupand with nothing treatment were blank control group. After2months period of ligation,through the following methods to evaluate the effect of the new antibacterial coating forprevention of early peri-implant infection.Method:1. After2months period of ligation, clinical measurement (mPI、PISF、mSBI、PD、MOB) and X-ray radiograph were measured.2. After2months period of ligation, Van Gieson (V-G) staining and scanning electronmicroscope (SEM) were carried out to observe the interface of implant-to-bone.3. After2months period of ligation, the pull-out test was carried out to observe thebonding strength of implant-bone interface.Results:1. The clinical measurement results showed that no significant difference was detectedbetween experimental group and positive control group, but the index of blank controlgroup was significantly higher than the above two groups (P<0.01). There was noobvious shadow detected by X-ray radiograph in experimental group and positivecontrol group, but significant shadow in blank control group.2. The implants combined with surrounding bone was well in experimental group andpositive control group observed by Van Gieson (V-G) staining and scanning electronmicroscope (SEM). The morphometry results showed that no significant difference was detected between experimental group （58.20±3.51%） and positive controlgroup（61.47±4.30%）, and blank control group（26.06±3.64%） was significantlylower than the above two groups (P <0.01).3. The bonding strength of implant-bone interface results showed that no significantdifference was detected between experimental group （2.40±0.51MPa） and positivecontrol group（2.45±0.81MPa）, and blank control group（1.83±0.64MPa） wassignificantly lower than the above two groups (P <0.01).Conclusion:The novel antibacterial coating, which containing halogenated furanone compounds,fabricated by our research group has remark effect for prevention of early implantpostoperative infection. |
PDF Full Text Request