Interventive Effects Of Purple Potato Anthocyanin On Exercise-induced Oxidative Damage In Myocardial Of Mice

Objective:Research shows that free radicals are the most direct material on mice,s cardiacoxidative damage and taking oxidizing substances can reduce myocardial oxidativedamage by reducing the produce of free radicals. In order to find a new substance whichcan resist cardiac oxidative damage and expand the source of substances that aboutresistance to cardiac oxidative damage, we take the antioxidant (purple sweet potatoanthocyanins) as the breakthrough point to analyzing the influence of antioxidant(purple sweet potato anthocyanins) on cardiac oxidative damage of overtraining mice.During this experiment, we plan to investigate the intervention of purple sweet potato inthe oxidation damage resulted from overtraining, and support a base for the furtherstudy of purple sweet potato intervention.Methods:Our study was based on8weeks-old male SD mice and before the formalexperiment we carried on our preliminary experiment. A mature experimentalmethodwas selected for the preliminary test and after experiment, drew blood from thesemice’s abdominalaortic to prepare the blood serum and then measured thetestosterone and Cortisol which are factors can be used for the judging of excessivetraining. Through analyzing these factors, we can determine the mature method forovertraining and then carry out the formal tests.84healthy male8weeks-old SD micewas divided into four groups, blank control group (C), normal exercise group (NS),overtraining group (OT), and overtraining group with anthocyanins (OTH), all sportsgroups of mice are divided into two kinds of execution state, named quiet executedcondition (after adding “one” show) and exhaustion executed condition (after adding“two” show). The sport groups of mice do exercise from Sunday to Friday and have arest on Saturday in six weeks. After the experiments the blood serum prepared fromabdominal aortic and hearts of these mice were stored at minus80degrees Celsius,respectively. At last, measured the value of serum cardiac troponin I, creatine kinaseisoenzyme (CK-MB), lipid peroxide (LPO) in myocardial mitochondria, superoxide dis- mutase (SOD), and calcium concentration in mitochondria and observed the HE staini-ng.Results:1There were no significant differences in the levels of SOD, MDA, calcium co-ncentration, serum testosterone, cortisol, lactate dehydrogenase, creatine kinase,creatine kinase isoenzyme, and cardiac troponin (I) between group NS and group NS24.Compared with group C, muscle fibers in group NS were thicker and more arranged.2Compared with group C and NS, the levels of SOD and serum testosterone ingroup OT were significantly lower while the levels of MDA, calcium concentration,cortisol, lactate dehydrogenase, creatine kinase, creatine kinase isoenzyme, and cardiactroponin (I) were significantly higher. Tissue biopsies showed that compared withgroups C and NS, myocardial fibers have undergone hypertrophy and muscle fibers arein pathological derangement in group OT,.3Compared with group C and NS, the levels of SOD and serum testosterone ingroup OTH and OTH24were lower while the level of MDA, calcium concentration,cortisol, lactate dehydrogenase, creatine kinase, creatine kinase isoenzyme, and cardiactroponin (I) were significantly higher. The levels of SOD and serum testosterone ingroups OTH and OTH24were higher than in groups C and NS, but were not significantwhile the levels of MDA, calcium concentration, cortisol, lactate dehydrogenase, crea-tine kinase, creatine kinase isoenzyme, and cardiac troponin (I) were lower and thedifference of calcium concentration and cardiac troponin (I) were significant. Tissue biopsies also showed a degree of pathological derangement but slighter than that of ingroup OT.Conclusions:1Moderate movement can improve the antioxidant ability of myocardial, causethe enlargement of myocardial fibers, arrange more dense and orderly, guide the hearttoward the direction of the health development, and protect myocardi umto a certainextent.2Overtraining damage defense systems for radicals of mice, destroye myocardialcell structure, excessive muscle fiber hypertrophy, enlarge and disarrangemyocard-ial fibers and damage myocardial tissue in a certain degree.3Purple sweet potato anthocyanins,s intervention can improve the overtraininginduced antioxidant ability, protect the myocardial cell structure, reduce the phenomenon of muscle fiber,s complex arrangement, reduce myocardial oxidative damage andpatholo-gical phenomenon caused by overtraining.4After24hours rest, the cardiac of normal exercise got effectively repairedafter some temporary faint damage; the mice of overtraining got some repaired, too, but the degree was very small; Purple sweet potato anthocyanins,intervention can acceleratethe recovery rate of oxidation hurt resulted from overtraining to a certain degree.