The Role Of Dihydroartemisinin In Hepatoma Cell HepG2Growth And Mcl-1Caspase-3Gene Expression In Vitro

Objective: To observe the influence in the growth and proliferation of HepG2cells and the expression of Mcl-1and Caspase-3gene after different concentrations ofdihydroartemisinin (DHA) treate human hepatoma cell line HepG2in vitro.Methods: Preparate different concentrations of DHA and treate HepG2cells.There are four groups in my study including different concentrations of DHA groups(DHA25μmol/L, DHA50μmol/L, DHA100μmol/L) and blank control group. Thecell proliferation was assessed by MTT and the cell apoptosis was detected by flowcytometry instrument in four groups of hepatoma cells. The expression levels ofMcl-1were detected by RT-PCR and Western blot, the expression levels of Caspase-3were detected by Western blot, respectively, in four groups of hepatoma cells.Results: The proliferation of cells was decreased and the cell apoptosis rate wasincreased after treated with DHA (P<0.01), and in a concentration-dependent manner.Compared with blank control group, the expression of Mcl-1mRNA and protein inexperimental groups were decreased, the expression of activated Caspase-3protein inexperimental groups were enhanced (P<0.01), and in a concentration-dependentmanner.Conclusion: DHA can decrease the proliferation of HepG2cells and enhancethe apoptosis of the cells through the pathway of inhibiting the expression of Mcl-1and activating the Caspase-3in vitro in a concentration-dependent manner.