Composition And Mechanism Of Formation Of Kidney Stones In Diabetic Rats At High Blood Sugar And Its Impactde By Irbesartan

Purpose:Understanding diabetes in high blood sugar is easy to form calcium oxalate kidney stones and Irbesartan effects on calcium oxalate kidney stone formation under diabetic hyperglycemia.Methods:Selected60male SD rats, mice aged8weeks and weighing240-280g. Randomly divided into four groups, each15. Namely:Normal SD rats group (A)、 Normal SD rats were given angiotensin Ⅱ receptor antagonist administered group (group B)、diabetic rats group SD (group C)、diabetic SD rats were given angiotensin Ⅱ receptor antagonist administered group (D group). SD rats were detected in24-hour urine oxalate (OX), calcium (Ca2+) and uric acid (UA) concentration After feeding for6months; Determination of angiotensin Ⅱ in renal tissue and hyaluronic acid (HA) levels. HE staining of paraffin sections of kidney renal pathological changes;By western blot detection of renal osteopontin (OPN) expression levels; Kidney tissue was observed buildup of calcium oxalate crystals by Von-Kossa staining and electron microscopy environment.Results1.24-hour urine uric acid:Group C was significantly lower than in group A (P <0.05), GroupDwas significantly lower than in group B (P<0.05), There was no significant difference between groups C and D(P>0.05), There was no significant difference between group A and group B (P>0.05).2.24-hour urine oxalate、calcium ion concentration:group C was significantly higher than in group A(P<0.05), group D was significantly higher than in group B(P <0.05), There was no significant difference between groups C and D(P>0.05), There was no significant difference between group A and group B (P>0.05).3. Angiotensin Ⅱ and hyaluronic acid content of renal tissue:Group C was significantly higher than in group A (P<0.05), Group D was significantly higher than in group B (P<0.05),Group B is lower than group A (P<0.05), D group was significantly lower than in group C (P<0.01).4. HE staining of paraffin sections of kidney renal pathological changes:Groups A and B showed no abnormalities. Group C in rat renal tubular epithelial cells was swelling, degeneration, necrosis, renal tubular dilation part, seen a lot of hyaline degeneration. Pathological changes and hyaline degeneration in group D than in group C to reduce.5.western blot detection of renal osteopontin expression levels:Osteopontin were expressed in the kidney of each group, Group B than in group A slightly weakened expression of osteopontin, D group was significantly decreased compared with group C protein osteopontin, C group osteopontin protein expression was significantly than Agroup, Group D than in group B increased osteopontin expression.6. Kidney tissue was observed buildup of calcium oxalate crystals by Von-Kossa staining and electron microscopy environment:Group A、B was not observed the presence of calcium oxalate crystals in the kidney tissues, Group C、D calcium oxalate crystals observed in the presence of renal tissue, Kidney tissue in group C than in group D accumulation of calcium oxalate crystals.Conclusions1.After a long period of time to maintain diabetes high blood sugar levels under24-hour urine oxalate, calcium ion concentration, urinary uric acid is reduced.2.irbesartan does not affect the24-hour urine oxalate, calcium and uric acid concentration.3. diabetes in high blood sugar levels and the formation of calcium oxalate kidney stones rather than an independent risk factor for uric acid stones.4. irbesartan can inhibit or delay diabetes in high blood sugar levels of calcium oxalate kidney stone formation.5.irbesartan mainly by reducing hyaluronic acid, the expression of osteopontin and reduced oxidative damage to kidney cells to Inhibit or delay diabetes calcium oxalate kidney stone formation in high blood sugar levels.