The Preliminary Study Of LZTFL1on Proliferation, Adhesion, Migration And Invasion Of Hepatocellular Carcinoma Cells

Objective:To investigate the effection of LZTFL1gene on proliferation, adhesion,migration and invasion in hepatocellular carcinoma cells.Methods:The MHCC-97H cells were cultured by using with high sugar unique antibioticDMEM containing10%of FBS. The MHCC-97H cells were transfected with theLZTFL1DNAs encoding plasmid by the Liposome. The MHCC-97H cells could bedivided into three groups: the transfection LZTFL1plasmid group,the transfectionempty vector group and the blank control group.1. Western blot method detected the protein expression of LZTFL1in the threegroups of cells.2. MTT method detected the change of proliferation in the three groups of cells.3. Artificial basement membrane adhesion test detected the change of adhesionin the three groups of cells.4. Transwell Chambers migration experiment detected the change of migrationin the three groups of cells.5. Transwell Chambers invasive experiment detected the change of invasion inthe three groups of cells.Results:1. Western blot method showed: the protein expression of LZTFL1of thetransfection LZTFL1plasmid group, transfection empty vector group, blank controlgroup were1.223±0.024，0.796±0.011，0.787±0.012.The protein expression ofLZTFL1of the transfection LZTFL1plasmid group had a significantly higher thanthe other two groups(P <0.05); the protein expression of LZTFL1of the transfectionempty vector group and blank control group had no obvious difference (P=0.533>0.05).2. MTT method showed: the absorbance value(A) of the transfection LZTFL1plasmid group, transfection empty vector group and blank control group were 1.487±0.025，2.036±0.071，2.120±0.089. The absorbance value(A) of the transfectionLZTFL1plasmid group had a significantly lower than the other two groups (P <0.05);the absorbance value(A) of the transfection empty vector group and blank controlgroup had no obvious difference (P=0.070>0.05).3. Artificial basement membrane adhesion test: the absorbance value(A) of theadhesive cells of the transfection LZTFL1plasmid group, transfection empty vectorgroup and blank control group were1.349±0.144，1.630±0.064，1.648±0.040. Theabsorbance value(A) of the adhesive cells of the transfection LZTFL1plasmid groupwas siginificantly lower than the other two groups (P＜0.05); the absorbance value(A)of the adhesive cells of the transfection empty vector group and blank control grouphad no obvious difference (P=0.761＞0.05).4. Transwell Chambers migration experiment showed: the number of cellsthrough the transwell Chamber of the transfection LZTFL1plasmid group,transfection empty vector group and blank control group were14.533±4.002，43.333±2.053，43.467±2.082. The number of cells through the transwell Chamber ofthe transfection LZTFL1plasmid group was obviously less than the other twogroups(P＜0.05); there was no obvious difference between the transfection emptyvector group and blank control group (P=0.956＞0.05).5. Transwell Chambers invasion experiment showed: the number of cellsthrough the transwell Chamber of the transfection LZTFL1plasmid group,transfection empty vector group and blank control group were1.067±0.503，7.333±1.474，7.400±1.000. The number of cells through the transwell Chamber of thetransfection LZTFL1plasmid group was obviously less than the other two groups(P＜0.05); there was no obvious difference between the transfection empty vector groupand blank control group (P=0.942＞0.05).Conclusion:LZTFL1gene could inhibit the ability of proliferation, invasion and metastasisof hepatocellular carcinoma.