Preparation Of Cell Microcarriers By Freeze-drying

Liver failure is a big challenging disease in clinic. Although liver transplantationcan save patients’ life, most of them died in the process of waiting for livertransplantation because of the shortage of liver donors. With the development of thebioartificial liver support system, the bioartificial liver is used for liver failure patientswith extracorporeal liver support method.The purpose of this paper is to explore a new preparation technology of the porousmicrocarrier for the biological artificial liver. Hepatoctyes were cultured on the surfaceof microcarrier to verify its biocompatibility.1. Mixing sodium alginate and N-carboxyl propionyl chitosan sodium into CS,sodium alginate/chitosan porous microcarriers and N-carboxyl propionyl chitosansodium(CPCS)/chitosan(CS) porous microcarriers were prepared by emulsionfreeze-drying method. Human liver cell line-02(L-02) were cultured on the surface ofthese microcarriers to test their bioactivity.2. Scanning electron microscope was used to analyze the microscopic structure ofthe microcarriers. The factors of the microcarriers as water absorption, degradation invitro were tested to evaluate their bio-properties. The cell proliferation anddifferentiation on the surface of porous microcarriers were analyzed by methyl thiazolyltetrazolium (MTT) assay.3. For the N-carboxyl propionyl chitosan sodium(CPCS)/chitosan(CS) porousmicrocarriers, the aperture and porosity of the freeze-dried microcarriers using pentanolas pore-foaming agent were3-55m and88%, respectively. However, the microcarriersusing pentanol and NH4Ac as pore-foaming agent were15-55m and94%, respectively.The two kinds of microcarriers had strong hardness and higher water adsorption. Themicrocarriers could biodegrade absolutely in vitro. Human liver cell line-02(L-02) grewwell on the surface of N-carboxyl propionyl chitosan sodium/chitosan microcarriers.4. For the sodium alginate/chitosan microcarriers, the aperture and porosity of thefreeze-dried microcarriers using pentanol as pore-foaming agent were3-50m and89%,respectively. However, the microcarriers using pentanol and NH4Ac as pore-foamingagent were15-55m and95%, respectively. The two kinds of microcarriers had stronghardness and the higher water adsorption. The microcarriers could biodegradeabsolutely in vitro. Human liver cell line-02(L-02) grew well on the surface of sodium alginate/chitosan porous microcarriers.In a word, in this paper the two kinds of microcarriers had good biologicalproperties, which can meet the requirements of the bioartificial liver on liver cellculture.