Study Of The Effects Of SiRNA Targeting LSD1Gene On The Proliferation And Apoptosis In Jeko-1Cell Line

Objective: To study the state of LSD1, H3K4me1，H3K4me2in mantle celllymphoma (MCL). We further study the effects of LSD1on cell proliferation,apoptosis and histone methylation of H3K4in Jeko-1cells.Methods：1.The expression of LSD1, and histone H3K4me1、H3K4me2in MCLdetected by Immunohistochemical.2. siRNA segment targeting LSD1gene wasdesigned and transfected into Jeko-1cells by LipofectamineTM2000. LSD1mRNAtranscription level was detected by RT-PCR.3. Cell proliferation curve was draw byMTT. Cell apoptosis was measured by Flow cytometry.4. The expression of Bcl-2,CyclinD1, C-myc and LSD1protein and histone methylation of H3K4me1, H3K4me2were detected by Western Blot.Results:1. The expression of LSD1was increased and histone methylation H3K4me1,H3K4me2were decreased in MCL compared to proliferative lymphadenitis, p＜0.05.2. LSD1mRNA and its protein were inhibited after transfected with LSD1siRNA.3. LSD1siRNA inhibited cell growth and induced cell apoptosis in dose andtime-dependent manner in Jeko-1cells line. IC50was60nM.4. The expression ofBcl-2, CyclinD1, C-myc was decreased after transfection for24hours.5. LSD1siRNA inhibited the expression of LSD1mRNA and upregulated histone methylationof H3K4me1, H3K4me2.Conclusions:1. LSD1might be one of the Pathogenesis in MCL since they are higherexpression which might result in low expression of H3K4me1and H3K4me2.2.LSD1siRNA inhibites cell growth and induces cell apoptosis in Jeko-1cell line.3. LSD1siRNA upregulates histone methylated H3k4me1and H3K4me2. It might be a newtherapeutic target in MCL.