Research On The Function Of C-myc And CyclinD1in The Pathogenesis Of Endometriosis

【Objective】This thesis aims to detect the expressions of estrogen target genes c-myc and cyclinD1in patients with endometriosis, and explore the possible correlation between them. Moreover, in order to identify the potential correlation of the expressions of estrogen target genes, i.e. C-myc and cyclinD1, and CA125of the patient, we investigate the difference between the detected expressions of c-myc and cylinD1in a group of patients with different stages of endometriosis.【Method】The treatment group consists of30EMS patients who, according to diagnostic standard, had maternal open or laparoscopic operations in the Child Health Hospital of Quanzhou City during the period from July2012to December2013. While the control group includes30patients who had operations due to tubal lesion during the same period. By applying According to the diagnostic standard, from July2012to December2013,open or laparoscopic operation for the treatment of maternal and Child Health Hospital of Quanzhou City,30cases of EMS patients as case group; the same period due to operation treatment of other benign diseases for patients30cases as control group. Using Immunohisto chemistry and real time PCR probe method, we detect the expressions of c-myc and cyclinD1in ectopic endometrium and peritoneum of the patients in the treatment group and compare them to these detected in the control group.【Results】:1、The comparison of general clinical data shows no statistically significant difference between the treatment and the control group.2、We detect the expressions of c-myc and cyclinD1in endometrium and peritoneum in both groups. However, compared to the control group, the c-myc and cyclinD1in the treatment group tend to be over-expressed. 3、Expressions of c-myc and cyclinD1in the endometrium and peritoneum of the individuals with different stages of ectopic endometrium are no evidently different.4、The expressions of c-myc and cyclinD1in the individuals in the treatment group are significant positively correlated.5、We find no evidence showing that there is a significant correlation between the expression of c-myc and cyclinD1and CA125in the treatment group.【Conclusion】1、The expressions of c-myc and cyclinD1in the ectopic focus tissue of endometrium and peritoneum in the treatment group are significantly higher than the control group.2、C-myc and cyclinD1are associated with endometriosis. In particular, the abnormal expressions of c-myc and cyclinD1accelerate the synergistic progress of cell G1-S, leading to a proliferation of cells.3、There is no correlation between the expressions of c-myc and cyclinD1and CA125in the individuals over all endometriosis stages in the treatment group.