| Objective: The purpose of this study was to investigate the role and expressionof HMGB1in intestinal tract injury of sepsis, and to discuss the mechanism of itstreatment with ethyl pyruvate, by establishing an experimental model ofsepsis-induced intestine injury in rats.Methods: Sixty male SD rats were randomly divided into three groups, the shamgroup(Group S), sepsis group(Group C), and ethyl pyruvate treated group(Group M).Sepsis-induced intestinal tract injury model was established by using cecal ligationand puncture (CLP). Group M received intraperitoneal injection of ethylpyruvate(40mg/kg) immediately after operation, while Group S and Group C wereboth given intraperitoneal injection of Lactated Ringer’s Solution. Five rats in eachgroup were picked up at3,6,12,24hour after CLP to have intestinal tractpathological damage observed by microscope, and the plasma levels of diamineoxidase (DAO) and D-lactic acid measured by spectrophotometry, and the HMGB1mRNA expression of small intestinal tissues detected by RT-PCR, and also the levelof small intestinal HMGB1measured by immunohistochemical method.Results:1. The pathological damage condition of intestinal tract: there was nosignificant intestinal tract injury at each time point in Group S, and no significantdifference in intestinal histological scores. In Group C and Group M, obviousintestinal tract injury appeared at12,24hour, and both intestinal histological scoreswere rising with increased postoperative time. In Group C, the intestinal histologicalscores were higher than that of Group S at6,12,24hour, while in Group M, thescores were higher that of Group S at12,24hour (p<0.05). Compared with Group C,the intestinal histological scores of Group M were significantly improved at24hour(p<0.05). 2. The plasma levels of DAO and D-lactic acid: there were no significantdifferences at each time point in Group S. While in Group C and Group M, the plasmalevels of DAO and D-lactic acid were increasing with the lapse of time, and higherthan that of Group S at each time point (p<0.05). The plasma levels of DAO andD-lactic acid of Group M were obviously lower than that of Group C at6,12,24hour(p<0.05).3. The expression level of small intestinal HMGB1mRNA: there were nosignificant differences in the level of small intestinal HMGB1mRNA at each timepoint in Group S. While in Group C and Group M, the level of small intestinalHMGB1mRNA was increasing with the lapse of time, and higher than that of GroupS at6,12,24hour (p<0.05). Compared with Group C, the level of small intestinalHMGB1mRNA in Group M decreased significantly at6,12,24hour (p<0.05).4. The expression of HMGB1in small intestinal tissue: there were no significantdifferences in the expression of HMGB1in small intestinal tissue at each time point inGroup S (p<0.05). While in Group C and Group M, the expression of HMGB1insmall intestinal tissue was increasing with the lapse of time (but not markedly at24hour in Group M), and higher than that of Group S at6,12,24hour (p<0.05).Compared with Group C, the expression of HMGB1in small intestinal tissue inGroup M decreased significantly at6,12,24hour (p<0.05).5.The correlation analysis of the expression of HMGB1and histological scoresin small intestinal tissue: there was a significant correlation between the expression ofHMGB1and histological scores in small intestinal tissue at6,12,24hour, and theircorrelation coefficients were0.740,0.614and0.905, respectively（P<0.05）.Conclusion: The degree of intestinal tract injury was associated with theHMGB1expression of intestinal tissues. Ethyl pyruvate could reduce sepsis-inducedintestinal tract injury, which was likely due to the inhibition of HMGB1expression inintestinal tissues. |
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