The Comparative Application Of Hormonal Method And Liquid Nitrogen Method In Modeling Of Femoral Head Necrosis In Dogs

Objectives: To compare the application of hormonal method and liquidnitrogen method in modeling of femoral head necrosis in animals, and toestablish a reliable canine model of the femoral head necrosis which canbe used in basic research and treatment.Methods: All12beagle dogs were randomly divided into three groups:in group A, they were taken intravenous injection of the ipopolysaccharide10ug/kg which were repeated after24h, and immediately the injections ofmethylprednisolone10mg/kg were lasted for2weeks, which was interval1weed, which was continued2week. B group were established by improvedliquid nitrogen frozen method. Group C were the control group. At6and12weeks, the general morphology, imaging, X-ray, CT, MRI, histopathologyand others were observed and the records were analyzed by SPSS.Results: Endotoxin combined hormone method (group A), liquid nitrogenfreezing method (group B) when the two groups in the first six weeks ofthe femoral head X, CT showed no abnormalities, MRI, pathology both showedearly signs of avascular necrosis, the bone trabecular area and empty pitrate were significantly different (P<0.05). The empty pit rate wassignificantly lower than that of group B at6weeks (P <0.01). Comparedwith group B, dogs of group A were more systemic drug reaction and poorrepaired of the osteonecrosis, and the empty pit rate increased with timeextended (P<0.05), also the difference of the bone trabecular area wasnot significant (P>0.05). In group B, the osteonecrosis degree wasprogressively alleviated with the experimental observation time(P<0.05).Conclusion: Canine non-traumatic avascular necrosis of the femoral head necrosis in animal models of early can be established by the combineduse of the endotoxin and methylprednisolone or liquid nitrogen method indogs. The liquid nitrogen frozen can induce a model of ANFH which is easyto build, repeat and control, and is more suitable for standardizedtreatment. The hormone combination model is closer to femoral headnecrosis and is more suitable for the cause, pathology, or a comprehensivestudy.