The Role Of SDF-1/CXCR4Axis In Functional Electrical Stimulation Improving The Neural Dysfunction Of Rats With Cerebral Infarction

Objective To observe effects of Functional Electrical Stimulation (FES) on5－bromodeoxy-dine(BrdU)、vascular endothelial growth factor （VEGF） and stromalcell-derived factor1（SDF-1）in rats’ cerebral infarction side. Exploring themechanism of FES promoting neurological function in rats with acute cerebralinfarction.Methods The rats with permanent middle cerebral artery occlusion(MCAO) wererandomly divided into sham-operation group, control group and FES group.FEStherapy stared form2days after surgery. Neurological Severity Scores(NSS) to assessthe degree of nerve function defect,5－bromodeoxy-dine(BrdU) positive cells in theSubventricular zone(SVZ) of cerebral infarction side were detected byimmunofluorescence. Besides, SDF-1and VEGF in infarction side were detected byELISA.Results1.Two days and seven days after surgery,the NSS scores had no significantdifferences between FES group and control group(P>0.05).The NSS scores of FESgroup was lower than that of control group14days after surgery (P<0.01). A gradualdecline in the NSS of FES group,there were statistically difference between the threegroups after surgery(P<0.01). The NSS scores of control group14days after surgerywas lower than that of2days and7days after surgery.2.Seven days and14th daysafter surgery,the SDF-1content of brain infarction side in FES groups were significantly higher than that in control groups and sham-operation groups(P<0.01),control groups were significantly higher than sham-operation groups(P<0.01). TheSDF-1content of control group14days after surgery was significantly lower than thatof control group7days after surgery.In FES groups and sham-operation groups,therewere no statistically difference between the two groups after surgery(P>0.05).3.Sevendays and14th days after surgery, VEGF content and SVZ’s BrdU positive cells ofbrain infarction side in FES groups were significantly higher than that in controlgroups and sham-operation groups(P<0.01), control groups were significantly higherthan sham-operation groups(P<0.01). In FES groups,BrdU positive cells14days aftersurgery was significantly lower7days after surgery(P<0.01), VEGF content14daysafter surgery was significantly higher than7days after surgery(P<0.01).In controlgroups, VEGF content14days after surgery was significantly higher than7days aftersurgery(P<0.01), BrdU positive cells had no statistically difference between7daysand14days after surgery(P>0.05).In sham-operaion groups,VEGF content andBrdU positive cells had no statistically difference between the two groups aftersurgery(P>0.05).Conclusion1.FES can significantly promotes neurological function in rats with acutecerebral infarction.2.FES may active SDF-1/CXCR4axis and raise the content ofSDF-1.3.FES can significantly promotes Neural stem cells(NSC) proliferation andangiogenesis in rats with acute cerebral infarction.4.FES can significantly promotesneurological function in rats with acute cerebral infarction, one of the importantmechanism was by activating SDF-1/CXC chemokine receptor4(CXCR4) axis topromote. NSC proliferation and angiogenesis.