Neuroprotective Effects And The Mechanism Of Curcumin On Global Cerebral Ischemia/Reperfusion Injury In Rats

[Objective]:This study is aimed to explore the protective effect and its mechanism of curcumin on rat global brain ischemia/reperfusion injury.[Methods]:1) Rat global brain ischemia/reperfusion injury modal:Twenty minutes globle cerebral I/R injury was conducted on Sprague-Dawley rats.2)Experimental groups:Sham groups; Ischemia/Reperfusion groups;Curcumin low dosage groups;Curcumin medium groups and Curcumin high dosage groups.3)HE staing was performing to observe the neuronal death7days after reperfusion.4)Immunohistochemistry:ICH was performed to evaluate the activated microglia and astrosytes3days and7days after reperfusion.5)The rats’survival rate was evaluated between each groups7days after reperfusion.6)MDA expression was evaluated3days after reperfusion to evaluate the tissue oxidative damage.7)ImmunohistochemistryFluorescent quatitive PCR:The inflammation cytokines expression was evaluated3days after reperfusion.[Results]:After the ischemia period, curcumin administration15minutes before the ischemia can protects against transient global I/R injury in a dosage-dependent manner and curcumin can significantly increased rats’survival rates and reduced I/R-redueed neuronal death, A medium dosage of curcumin(50mg/kg) can reduced I/R induced glial activation3days and7days after reperfusion and inflammation cytokine overexpression in the hippocampus area3days after reperfusion. At the same time, curcumin can reduced the I/R-induced MDA overexpression of the hippocampus area3days after reperfusion.[Conclusions]:These resusts suggests that the neuroprotective potentials of curcumin against global ischemia/reperfusion injury are, at least in part, ascribed to its anti-inflammation effects.