The Effect Of Galectin-3Silencing On Cell Growth And Chemosensitivity In Gastric Cancer Cell Line SGC-7901

Objective: To investigate the changes of cell proliferation, apoptosis andchemosensitivity of gastric cancer cell line SGC-7901after transfected by Galectin-3smallinterfering RNA (siRNA).Methods: According to the sequences of coding regions NM-002306of Galectin-3inGenbank and the relevant literature, synthesized the Galectin-3siRNA and the negativecontrol siRNA, transfected gastric cancer cell line SGC-7901. The experiment was dividedinto four groups: blank control group, liposome group, negative siRNA group andGalectin-3siRNA group. The expression of Galectin-3at mRNA level at24hours aftertransfection was evaluated by Real time PCR, and the expression at protein level at72hours was evaluated by Western blot. The cellular proliferation inhibition rate was detectedby CCK-8method at24,48and72hours after transfection. Cell apoptosis was analyzedby AnnexinⅤ/PI-labeled flow cytometric analysis. After cell transfection, each group wasdisposed with different concentrations of Oxaliplatin（5、10、20、40、80ug/ml）, thencultured for48hours, detected by CCK-8method.Results: The transfection efficiency detected by flow cytometry at24hours aftertransfected with fluorescently labeled siRNA was83.8%, was fit for the requirements ofsubsequent experiments.△Ct value represented the expressions of Galectin-3at mRNAlevel, Galectin-3siRNA group was reduced by87.8%than the blank control group（P＜0.05）. The gray value ratio of Galectin-3and GAPDH represented the expressions ofGalectin-3at protein level, Galectin-3siRNA group was decreased by90.4%than theblank control group（P＜0.05）. However, the liposome group and negative siRNA groupshowed no significant difference compared to the blank control group（P＞0.05）. Thisdemonstrates that we successfully inhibited the expression of Galectin-3at mRNA andprotein levels by Galectin-3siRNA transfection. The cellular proliferation inhibition ratesof Galectin-3siRNA group at24,48and72hours after transfection were（15.57±1.45）%, （32.90±0.76）%and （57.35±1.05）%, the difference was statistically significantcompared with the rest of the control group（P＜0.05）. The apoptosis rate at72hours aftertransfection，the blank control group was（21.87±1.36）%, the liposome group was（24.67±1.40）%, the negative siRNA group was（27.73±2.55）%, and the Galectin-3siRNA group was（46.17±2.39）%. The last was higher than the other three control groups,the difference was statistically significant（P＜0.05）. The blank control group was treatedonly with different concentrations of Oxaliplatin（5、10、20、40、80ug/ml）, the cellularproliferation inhibition rates at48hours were （13.30±0.21）%,（26.82±0.55）%,（34.72±0.47）%,（41.32±0.05）%,（50.28±0.13）%. The Galectin-3siRNAgroup wastreated both with Oxaliplatin and Galectin-3siRNA, its cellular proliferation inhibitionrates were （19.94±0.61）%,（40.04±0.14）%,（62.04±0.13）%,（81.13±0.68）%,（90.59±0.68）%, were significantly increased（P＜0.05）. It showed the chemosensitivityto Oxaliplatin of SGC-7901had a significant increase after transfected with Galectin-3siRNA.Conclusion:1. We successfully inhibited the expression of Galectin-3in SGC-7901at mRNA andprotein level by Galectin-3siRNA transfection.2. When the expression of Galectin-3in SGC-7901was reduced, cell proliferationdecreased and cell apoptosis increased.3. When the expression of Galectin-3in SGC-7901was inhibited, chemosensitivityto Oxaliplatin of SGC-7901augmented.