SHOX2、GSTP1and RASSF1A Study On Methylation Detection In The Diagnosis Of Prostate Cancer

Background Prostate cancer (PCa) is a malignant tumor with the highest incidencerate of developed countries in Europe and America, mortality is also higher. PCa inthe incidence of a disease of our country was lower than those of Europe and theUnited States, but in recent years, the incidence of PCa in our country present obviousrising trend. For the early detection of patients with focal PCa for PCa radical surgeryis the best treatment, after the more ideal. PCa test goal is to diagnosis in the earlystages of the disease. Therefore, searching for PCa early tumor markers becomesurgent problems of urology clinical physicians. At present, the serum prostate specificantigen (PSA) as a single test indexes of PCa, although has the high PCa positivediagnosis rate of prediction, but it is influenced by age, prostate massage, piercing,infection, drugs, and the influence of such factors as its diagnostic sensitivity andspecificity is not high, can not fully reach the needs of PCa in early diagnosis, thus,early diagnosis in urgent need of PCa to sensitive specific biomarkers. Epigeneticsplay an important role in the development of tumor, abnormal DNA methylation isone of the most important way of modification, DNA methylation does not changethe sequence of nucleotides, but by changing the chromosome structure and the levelof histone acetylation, indirectly lead to inhibition of gene transcription. Abnormalmethylation is an early event in tumorigenesis, so the detection of DNA methylationcan become malignant tumor markers for early diagnosis value. Tumor is moregenetic variation process of accumulation, therefore, we believe that a variety of jointdetection of methylation gene can make up the defects of single gene detection. According to this purpose, we chose SHOX2screening, GSTP1and RASSF1Amethylation specific PCR in three genes joint detection and analysis of the optimalcombination of the two genes joint detection, improve the positive rate in earlydiagnosis of PCa, mining value in early diagnosis of PCa.Methods This topic collection of47cases of PCa paraffin organization and27cases of prostatic hyperplasia (BPH) paraffin tissue, methylation specific PCR (MSP)method is used respectively to SHOX2, GSTP1and level of methylation ofRASSF1A these three tests. Analysis of RASSF1A two SHOX2, GSTP1and jointdetection in the early diagnosis of PCa.Results PCa tissue SHOX2, GSTP1and RASSF1A gene methylation rate were17.02%,51.06%and65.96%respectively. BPH in40.74%,3.70%and22.22%respectively. PCa in GSTP1and RASSF1A gene methylation positive rate werehigher than in the BPH (P <0.05), while SHOX2gene methylation positive rate islower than in the PCa BPH (P <0.05). Compare two genes joint (SHOX2+RASSF1A, SHOX2+GSTP1, GSTP1+RASSF1A) methylation positive rate were70.21%,53.19%and87.23%respectively. Were higher than that of a single genemethylation positive rate (P <0.05).Conclusion1. This research shows that SHOX2in the occurrence and development of PCaproto-oncogene role.2. GSTP1+RASSF1Amethylationdetection sensitivity,detection ofGSTP1+RASSF1Ais expectedto bea biomarkerforearly diagnosisofPCa.