| Objective:Rheumatoid arthritis is a kind of chronic general auto-immuned disease which is charactered with synovitis in joints. The fundamental pathological change is chronic inflammation of the joint synovial leading to pannus, which destroys cartilage, bone and surrounding tissues. It shows that the infiltration and exudation of lymphocyte is the key factor in rheumatoid arthritis and T lymphocyte is dominated. According to the different functions, T cells can be divided into T helper cells (T helper cells, Th) and T suppressor cells (T suppressor cells, Ts). Th cells according to their different functions and the secretion of cytokines are divided into different subsets. All Th cells are made up of original CD4+T cells differentiation. Cytokines are the important regulatory factors in RA. The imbalance of proinflammatory factor and anti-inflammatory factor in rheumatoid arthritis leads to the autoimmune disease. In order to research the effect of IL-17and IL-10in rheumatoid arthritis, the expression and distribution of IL-17and IL-10in synovial membrane cells and synovial tissue were detected using immunohistochemical method.Methods:(1)In this retrospective study, immunohistochemical staining for IL-17and IL-10was performed in41cases of RA in Tianjin hospital(from2011to2013). Among these cases,31were females and10were males, and age ranges from20to72, the average age was53.7years old,32occured in knee joints,4in hip joints,1in elbows joints,3in ankle joints, and1in wrist joint. Besides,10cases OA were chosen as control group, age ranges from43to68, the average age was58.5years old.(2)All cases mentioned above which were fixed by neutral buffered formalin, embedded by paraffin, section thicked4μm. They were performed using immunohistochemical stain to detect the expression of IL-17and IL-10respectively.(3)Statistics analyse the data by SPSS17.0. The positive rates of IL-17and IL-10in RA and OA were compared with chi-square test. The expressive intensities of IL-17and IL-10in RA and OA were compared with rank sum test. Spearman rank correlation test was used to evaluate the correlation of expression between IL-17and IL-10. Results:(1)The positive rates of IL-,17in synovium of RA and OA were85.7%(36/41) and60.0%(6/10) respectively. The positive rate of IL-17was significantly higher than that of OA (P<0.05). The expressional rates of IL-10in synovium of RA and OA were53.7%(22/41) and60.0%(6/10). The expression of IL-10was lower than that of OA (P>0.05), but there was no monkedly difference in statistics.(2)The positive rate of IL-17in lining cell of RA was85.4%(35/41), and the expression of it in the subsynovial tissue of RA was87.8%(36/41), and the latter was higher than the former (P>0.05). The positive rate of IL-10in lining cell of RA was36.6%(15/41), and the expression of it in the subsynovial tissue of RA was53.7%(22/41), and the latter was higher than the former (P>0.05).(3)By Spearman rank correlation test, the expression of IL-17was negatively correlated with that of IL-10.Conclusion:(1)The positive rate of IL-17was higher than that of OA. IL-17may play an important role in the RA pathogenesis. The expression of IL-10in synovium was not significantly different between RA and OA. However, the positive rate of IL-10was lower than that of IL-17, which may decrease the protection of IL-10and promote the development of RA.(2)There was no significant difference between the expression of IL-17and IL-10in both synovium and subsynovial tissue of RA. It suggests that the expression of IL-17and IL-10have no depot difference.(3)The expression of IL-17was negatively correlated with that of IL-10. Herein, it suggests that the imbalance of IL-17/IL-10expression probably plays a certain role in the pathogenesis of rheumatoid arthritis. |
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