Changes Of Circulating Endothelial Progenitor Cells After Percutaneous Coronary Intervention Within24h In Patients With Coronary Heart Disease

Objectives:To observe the dynamic changes of CD34+cells、KDR+cells、 CD133+cells、CD34+/KDR+cells、CD34+/KDR+/CD133+cells、CD34+/KDR+/CD133-cells within24h after PCI, and to explore the effect of PCI on the number of which and the role in coronary endothelial damage repair.Methods:31cases with suspected unstable angina undergoing elective CAG were divided into2groups intervention group (n=22) and non-PCI group(n=9).Take2mL blood taken from the cases of intervention group at time of0h、1h、3h、5h、7h、24h after the first balloon expansion, from the cases of non-PCI group at the time of0h、7h、24h after CAG. The peripheral blood were used to detect the numbers of CD34+cells、KDR+cells、CD133+cells、CD34+/KDR+cells、CD34+/KDR+/CD133+cells、CD34+/KDR+/CD133-cells.Results:1.The number of CD34+cells in the intervention group increased at7h after PCI,then significantly increased at24h,The number of CD34+cells increased at24h after PCI by71%compared to Oh (P<0.05) and by29%compared to7h (P<0.05);However,the numbers of CD34+cells after cag did not show a statistical difference in the non-PCI group;The number of CD34+cells of non-PCI group is significantly higher than PCI group at0h(P<0.01);The number of CD34+cells between the non-PCI group and PCI group did not show a statistical difference at7h、24h.2. The number of KDR+cells of intervention group decreased to the lowest level at7h by94%compared to Oh (P＜0.01), and the number of KDR+cells at24h is higher than that at7h after PCI,which did not show a statistical difference; The number of KDR+cells after cag don’t show a statistical difference in the non-PCI group;The number of KDR+cells of PCI group is significantly higher than non-PCI group at Oh (P＜0.01); The number of KDR+cells at7h between the non-PCI group and PCI group did not show a statistical difference; The number of KDR+cells of PCI group is significantly higher than non-PCI group at24h (P＜0.05).3.The number of CD133+cells increased at24h after PCI by109%compared to Oh (P＜0.01);The number of CD133+cells after cag don’t show a statistical difference in the non-PCI group;The number of CD133+cells of PCI group is significantly higher than non-PCI group at0h、7h、24h (P＜0.01).4.The number of CD34+/KDR+cells increased at24h after PCI by61%compared to0h(P<0.01);The number of CD34+/KDR+cells did not show a statistical difference after cag in the non-PCI group;The number of CD34+/KDR+cells between the non-PCI group and PCI group did not show a statistical difference at0h、7h、24h.5.The number of CD34+/KDR+/CD133+cells at different time points within24h after PCI did not show a statistical difference;The number of CD34+/KDR+/CD133+cells of non-PCI group is significantly higher than PCI group at0h、7h、24h(P<0.01); The number of CD34+/KDR+/CD133+cells don’t show a statistical difference after cag in the non-PCI group.6.The number of CD34+/KDR+/CD133-cells within24h after PCI did not show a statistical difference;The number of CD34+/KDR+/CD133-cells at0h、7h、24h between the non-PCI group and PCI group did not show a statistical difference.Conclusions:1. Patients with severer CHD have higher level of KDR+cells and lower level of CD34+cells. CD133+cells. CD34+/KDR+cells. CD34+/KDR+/CD133+cells,However, the number of CD34+/KDR+/CD133+cells between the non-PCI group and PCI group did not show a statistical difference.2.Endothelial injury induced by PCI promoted the mobilization、migration、differentiation、homing of the CEPCs,and promoted the mobilization、migration of CD34+cells、CD133+cells and CD34+/KDR+cells,causing a decline of the number of KDR+cells,however the number of CD34+/KDR+/CD133+cells. and CD34+/KDR+/CD133-cells did not show a significant change after PCI.