| ObjectiveBreast cancer is one of the most common malignant tumor of women, threatens women’s health. Breast cancer is a hormone-dependent cancer, and the study of related genes is the hot spot of molecular biology research. Estrogen is an important factor about pathogenesis and treatment of breast cancer, and also is an important factor that affect women bone density. In postmenopausal women, low estrogen level affect bone density. The study showed that increase bone mineral density in postmenopausal women increased the risk of breast cancer, bone density may be used as an indicator to predict breast cancer risk. C6orf97is located upstream of the ESR1gene open reading frame, and C6orf97is a bone density-related genes. We might speculate there is a regulating effect between the gene C6orf97and ESR1, and it is an important gene associated with breast cancer. The aim of this study is to explore the factors on bone mineral density in postmenopausal breast cancer patients and the correlation of C6orf97and ESR1and bone density. We hope to find a new starting poin about breast cancer research, prevention and treatment.MethodsSelect106breast cancer patients from the Tianjin Medical University Cancer Hospital during October2008to July2013by histopathological diagnosis and surgery. All patients were post-menopausal women, estrogen receptor-positive patients were received endocrine therapy. All patients underwent bone density testing every six months to evaluation and follow-up. Take18cases of breast cancer tissue in breast cancer tissue repository for gene expression analysis. By extracted tissue RNA, we used real-time PCR to detect the expression of C6orf97and ESR1, and then analyze the correlation of these two genes and bone density.Results1. All patients underwent bone density testing. The average of lumbar spine BMD is1.00±0.16g/cm2, femur neck bone mineral density is0.93±0.13g/cm2. The results of descriptive analysis of bone mineral density of the lumbar spine and femur neck bone mineral density in different age groups show:among different age groups systemic bone mineral density differences are meaningful (P=0.001). The factors of BMI (P=0.038), reproductive history (P=0.032), p53(P=0.019) on the lumbar spine bone mineral density are statistically significant. The factor of BMI (P=0.049) also affect femur neck bone mineral density is statistically significant. The correlation analysis showed that BMI (r=0.196, P=0.045), p53(r=0.321, P=0.002) was positively correlated with lumbar spine BMD. Age of diagonosis (r=-0.359, P=3.100×10-4), the years after menopause (r=-0.374, P=1.576×10-4) were negatively correlated with femur neck BMD value; and BMI (r=0.219, P=0.031), p53(r=0.223, P=0.039) were positively correlated with femur neck BMD value.2. The expression of ESR1and C6orf97expression is highly correlated (r=0.699, P0.001). The correlation analysis shows that the expression of PR gene and C6orf97gene is also positively correlated (r=0.672, P=0.002).3. The Log-rank survival analysis showed that surgical procedures affect patients OS (P=0.014) and DFS (P=8.585×10-9), and lymph node metastasis (P=0.004) and pathology stage (P=0.003) affect patients DFS. The Cox Multi-factor analysis showed that lymph node metastasis was an independent factor of DFS (RR=0.672, P=0.035).Conclusions1. Age of diagonosis, BMI, reproductive history, the years after menopause and the status of p53are related factors affecting bone mineral density in postmenopausal breast cancer patients.2. The expression of C6orf97are highly correlated with ESR1, there may be some relationship between them. The correlation analysis also showed that there is a relationship between C6orf97and PR. BMD and gene C6orf97may be a new direction for the treatment of breast cancer research.3. The surgical procedures affect patients DFS and OS, and lymph node metastasis and pathology stageaffect patients DFS. The Cox Multi-factor analysis showed that lymph node metastasis was an independent factor of DFS. |
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