The Expression Of Wnt1Protein And β-catenin、APC Protein And Gene In Breast Carcinoma And Their Clinicopathologic Significance

Breast cancer is the most common malignant diseases in women all overthe world,the infiltrating ductal carcinoma of the breast is the most commontype of breast cancer.In recent years, the incidence of breast cancer is rising,in some big city in China has grown to be the the first female cancer.The mostcommon cause of death in patients with breast cancer is metastasis andrecurrence,however,the pathogenesis of breast cancer is not very clear,recentstudies suggest that Wnt signaling pathway plays an important role in thepathogenesis and progression of human breast cancer,nearly50%of the tumorformation was related to the classic Wnt signal pathway activation.Wnt1is thefirst member of the Wnt signal transduction pathway,abnormal expression ofwhich was found in many tumors,but research situation about Wnt1expression in breast cancer were few both at home and abroad.Wnt/β-cateninsignaling pathway is an important part of the Wingless signaling pathway,changing the path will lead to abnormal expression of β-catenin cells,activation of downstream target genes,thus starting the tumor growth program.APC protein binds to Wnt pathway of Axin, GSK-3β,β-catenin and otheringredients,can stimulate the β-catenin was GSK-3β phosphorylation anddegradation of β-catenin,leading to beta.If the lack of APC protein,β-cateninwill not degrade too much and in the nucleus of the aggregation,which led tocell division.So,the deletion of APC gene may influence between cell growthand cell death in the balance.This study was to detect the expression of Wnt1protein and β-catenin,APC protein and gene in normal breast tissue,breasthyperplasia and breast invasive ductal carcinoma,and its relationship with clinical pathological characteristics,and the correlation between Wnt1andβ-catenin,between APC and β-catenin,aims to explore the effect of them in thedevelopment of breast cancer.Objective:Discussion on the development of infiltrating ductal breast cancer Wnt1protein and β-catenin,APC protein and gene function and its relationship withclinical pathological characteristics;relationship between β-catenin and Wnt1,APC in breast invasive ductal carcinoma;the relationship between APC proteinand gene expression of β-catenin.Methods:Select a block of wax breast disease specimens of120patients betweenJanuary2010to December2012in the251Hospital of Chinese PLA,included15cases of normal breast tissues (breast normal control group),25cases of breast hyperplasia tissues (breast hyperplasia group),80cases ofbreast invasive ductal carcinoma (breast cancer group),tissue specimens wereroutinely fixed by the Formaldehyde Solution,paraffin embedding,serialsections of4μ m,65℃oven baked slices overnight,in case of off chin.Immunohistochemistry was performed to detect the expression of breastcancer S-P method and in situ hybridization side group,breast hyperplasia,breast cancer group of Wnt1protein and β-catenin,APC protein and gene.SPSS17.0statistical software was used for analysis,count data by χ2test,correlation analysis using Spearman test,to test the level of a=0.05,agreementanalysis by Kappa test.Results:The positive expression of Wnt1in breast normal control group,breasthyperplasia group and in breast cancer group were:13.33%(2/15),28%(7/25),62.5%(50/80),the difference was statistically significant(P<0.01).Abnormalexpression of β-catenin in breast normal control group,breast hyperplasiagroup and in breast cancer group were:13.33%(2/15),20%(5/25),68.8%(55/80),the difference was statistically significant(P<0.01).The positiveexpression of APC in breast normal control group,breast hyperplasia group and in breast cancer group were:93.3%(14/15),80%(20/25),45%(36/80),thedifference was statistically significant(P<0.01).The positive expression ofβ-catenin protein and gene in breast normal control group were:13.33%(2/15),13.33%(2/15),the positive expression in mammary gland hyperplasiagroup:20%(5/25),24%(6/25),the positive expression in the breast cancergroup:68.8%(55/80),65%(52/80),there were no significant differences(P>0.5).The positive expression of APC protein and gene in breast normalcontrol group was:93.3%(14/15),93.3%(14/15),positive expression inmammary gland hyperplasia group was:80%(20/25),72%(18/25),positiveexpression in breast cancer group were:45%(36/80),43.8%(35/80),there wereno significant differences(P>0.5).The abnormal expression and lowexpression of positive expression of APC,Wnt1in breast infiltrating ductalcarcinoma of β-catenin were associated with lymph node metastasis (P<0.05),β-catenin expression and low expression of APC protein also infiltratingductal carcinoma and breast histological grading (P<0.05).In breast invasiveductal carcinoma and expression of β-catenin and Wnt1protein wassignificantly positive correlation(r=0.313, P<0.05).Expression of β-cateninand APC protein was significantly negative correlation (r=0.313, P<0.05).Nostatistical significance between the expression of β-catenin and β-cateninmRNA protein,APC protein and APC mRNA (P>0.5).Conclusion:The expression of β-catenin was positively correlated with Wnt1,andnegatively correlated with APC, There has no difference in protein level andgene level description of β-catenin and APC. Wnt1、β-catenin and APC takepart in the occurrence and development of breast cancer,closely related tolymphatic metastasis, can be used as a marker to evaluate the prognosis ofbreast cancer.