The Anti-tumor Effects Of Dendritic Cells And Cytokine-Induced Killers Cells Co-cultured Combination With Chemotherapy Against Lung Carcinoma In Vitro

Objective:Currently， lung carcinoma is one of the most common malignanttumor,its incidence ranks first and mortality fastest growing in themalignancies.Lung cancer divided into non-small cell lung cancer(NSCLC)and small cell lung cancer (SCLC) and NSCLC accounts for about85%of thetotal incidence of lung cancer. Most patients found already late, and oftenassociated with malignant pleural effusion, although the traditional treatmentssuch as surgery, radiotherapy and chemotherapy in the growing，but there stillhave some limitations，and lung cancer’s interim survival have no noticeableimprove. With further research on the pathogenesis of cancer,biologicalimmune technology continues to evolve, biological therapy has become animportant method for the treatment of malignant tumors, We are concernedabout dendritic cell (DC) because it is the body’s most powerfulantigen-presenting cells and initiator of anti-tumor immune response.Dendritic cell and cytokine-induced killers cells(CIK) co-cultured cellsproliferating fast，deadly characteristics was the focus of tumor biotherapyresearch.This study aims to obtain mature dendritic cells from Lung cancerpatients’malignant pleural effusions,to establish the effect of malignant pleuraleffusion derived dendritic cells co-cultured with cytokine-induced killers cellscombined with chemotherapy for lung cancer cell,provide new avenues for thetreatment of patients with advanced lung cancer.Method：20patients in the department of our hospital with Non-small cell lungcancer and pleural effusion form September2012to October2013were closen,800-1000ml fluid collected in each case. Double density gradientcentrifugation and adherent cells to obtain pleural effusion dendritic cellsprecursor cells,and cultured for9days in medium with granulocytemacrophage colony-stimulating factor(GM-CSF)、plus interleukin(IL-4)、TNF-α.CIK cells were perpared routinely from human peripheral bloodmononuclear cells(PBMC) and cultured with cytokines.DC and CIK cellsco-cultured on the nine day to get DC-CIK cells.Flow cytometry was used toexplore the immunophenotypes,and in vitro cytotoxicity was examined byMTT assay.Result：1. DC of malignant pleural effusion had the typical morphology afterextracorporeal cultivation,and the phenotype of DC generated from effusionshowed higher expression of HLA-DR（70.97±8.96）%、CD8（056.61±7.01）%、CD83（58.85±7.05）%、CD86（60.27±13.94）%than the control group(P＜0.05).2. CIK cells derived from human peripheral blood，and after co-culturewith malignant pleural effusion sources DC to obtained DC-CIK cell.The maineffect of DC-CIK cells CD3+CD56+cells were significantly enhanced thanthat of the CIK cultured alone(P＜0.05).and the killing effects of CIK werealso obviously increased(P＜0.05).3. DC-CIK cells combined with oxaliplatin obviously inhibited thegrowth of lung adenocarcinoma cells in vitro than monotherapy group(P＜0.05).Conclusion：Pleural effusion source of DC precursor cell cultured in vitro maturationavailability functioning DC, CIK cells were co-cultured with DC can promoteCIK cells proliferation and enhance anti-toxic.DC-CIK cells combined withoxaliplatin obviously inhibited the growth of lung adenocarcinoma cells invitro, To provide a theoretical basis for the comprehensive treatment of lungcancer.