Research Pill Intervention Xifeng Flutter Mechanism Of Apoptosis In Rat Cells In Parkinson’s

Objective：To observe the effect of calming wind behavior fibrillation Pill on ratmodel of Parkinson disease and apoptosis related gene Bcl-2, Bax, Caspase3proteincontent, and then the xifengdingchan pill in rat models of Parkinson disease behavioranalysis studies and apoptosis of dopaminergic nerve yuan, from the protection pointof view to provide a theoretical basis for the treatment of Parkinson’s disease withtraditional Chinese medicine..Methods：The model of Parkinson’s disease with the rat substantia nigra striatuminjection of6-hydroxydopamine (6-OHDA) were prepared by the method of. Healthyrats were chosen as blank group. The successful model rats were randomly dividedinto xifengdingchan pill model group (model group), xifengdingchan pill low dosegroup (low dose group), xifengdingchan pill (low dose group, middle dose group)xifengdingchan pill high dose group (high dose group), beauty DOPA group. Everyday to give the corresponding drug or water feeding, were fed for4weeks, duringapomorphine induced behavior of rats were observed and recorded the change.Immunohistochemistry was used to determine the expression of Parkinson’s diseasemodel rat substantia nigra striatum dopaminergic neuron apoptosis related proteinBcl-2, Bax and Caspase3method.Results：1, change the behavior of rat model of Parkinson disease: changing therotational behavior of blank group, low dose group rotation frequency is changed, butcompared with the model group had no statistical significance (P>0.05). The middledose group and high dose group, model group and Madopar group, a rat model ofParkinson disease in rotating frequency change had no statistical significance (P>0.05), no significant difference in high dose group and Madopar group (P>0.05).2,the expression in a rat model of Parkinson disease substantia nigra striatum of Bcl-2,Bax and Caspase3protein: low dose of Bcl-2group compared with the model group,the difference was statistically significant (P<0.05) compared with the model group, the expression of Bax and Caspase3protein, there was no statistical significance (P>0.05). Compared with middle dose group, high dose group, were statisticallysignificant (P<0.05or P<0.01). The middle dose group compared with the modelgroup and blank control group, the expression of Bcl-2, Bax and Caspase3proteinwere changed, the difference was statistically significant (P<0.05), compared with thehigh dose group, the expression of Bcl-2and Bax protein had a significant difference(P<0.05). The protein expression of Caspase3had no significant difference (P>0.05).Expression compared with model group and high dose groups of Bcl-2, Bax andCaspase3proteins, were statistically significant (P<0.05or P<0.01), there were nostatistical differences in Bcl-2, Bax and the control group (P>0.05), Caspase3and thecontrol group had significant difference (P<0.05). Expression compared with theblank group and the model group Madopar group Bcl-2, Bax and Caspase3proteins,were statistically significant (P<0.05) or (P<0.01).Conclusion：Xifengdingchan pill can reduce a small enough in a rat model ofParkinson disease apomorphine induced rotation number.2, xifengdingchan pill byenhancing the expression of Bcl-2protein, decreased Bax and Caspase3proteinexpression to inhibit the apoptosis of dopaminergic neurons.3, xifengdingchan pillhas certain dose effect relationship and cell apoptosis in a rat model of Parkinsondisease behavior change.