| Objective: Concept Chamai Asp soup on human skin fibroblasts(HSAS1)proliferation and collagen synthesis activity,investigatesystemi cscleroderma(SSc)TCM treatment of new ideas, also Chinese,"Lung relatingto skin" theory provides reference.Methods:①preparation containingserum and western medicine: the60SDrats were randomly divided into groups Ophiopogon soup, medicine controlgroup, nor mal control group20. Ophi opogon soup rats to Ophiopogonis4.24g/100g/d,(click here to calculate the concentration of crude drug per1ml liquid containing crude drug4.24g) once a day orally; Angelica fourrats in the control group inverse soup2.44g/100g/d (click here to calculatethe concentration of crude dr ug per1ml liquid containing crude drug4.24g),once daily orally; nor mal control group rats given doses Ophiopogon soupgroup with the same volume of physiological saline gavage, the threegroups were fed for a week. Three groups of rats6h after the lastadministration of abdominal aortic blood, centrifuged supernatant, afterinactivation, sterile filters, packaging,-70℃t o save backup. Thin Chigroup (600μg/ml thin broth glycopeptide Chicago):Take a thin Chiglycopeptide injection3,(each2ml, containing5mg polysaccharides:1mgpeptide) was added24ml DMEM high glucose complete medium, dubbed600μg/ml Chiglycopeptide thin broth,4℃to save backup.(Dose based onclinical weight60kg adult dosage6mg/kg/d projections).②HSAS1cultured cells.③serum containing glycopeptide and thin Chi interventionHSAS1cells.④MTT assay Ophiopogonsoup for HSAS1proliferationactivity.⑤by flow cytometry and AO/EB staining were used to det ect theimpact of HSAS1Ophiopogon soup apoptosis.⑥by quantitative PCR method to detect the impact of Ophiopogon soup Ⅰcollagen mRNAexpression.Results:①invertedmicroscope, Ophiopogon soup of high, medium andlow-dose serum HSAS1no significant differ ence in changes in cellmorphology.②di fferent ti mes, Ophiopogon soup low, medium and highdose groups HSAS1OD absorbance value is lower than the nor mal controlgroup, the difference was statistically significant (P<0.01); Angelica SNDlow, medium and high dose groups HSAS1absorbance OD value i s lowerthan the nor mal control group, the di fference was statistically significant(P<0.01); Ophiopogon soup Angelica high-dose group and high dose groupHSAS1absorbance OD value of the mini mum, no significant differencebetween the two groups compared (P>0.05).③thin Chi group HSAS1ODabsorbance values36h,48h lower than the normal cont rol group, thedifference was not statistically significant (P>0.05); thin Chi glycopeptideHSAS1absorbance OD val ues higher than medicine serum group, thedifference was statistically significant(P<0.05).④with increasing serumconcentrations of serum HSAS1Ophiopogon soup proliferation inhibitionrate increased;Dangguisini soup with increasing serum concentrations ofHSAS1proliferation inhibition rate increased;thin Chi glycopeptide HSAS1no proliferation inhibition ratesignificantly affected.⑤Ophiopogon soupserogroup HSAS1apoptotic index was lower than the nor mal controlgroup,the difference was statistically significant (P<0.05).⑥each treatmentgroup Ⅰskin fibroblasts and collagen mRNA expression were lower than thecontrol group, the difference was significant (P<0.01).⑦Ophiopogon soupwith Angelica high-dose serum reduces the skin to high doses of serumcollagen type ⅠmRNA expression of fibroblasts, compared with the thinChi glycopeptide differences (P<0.01).Conclusion:①with increasing serum concentration and time, Ophiopogonsoup sera sera Dangguisini soup into human skin fibroblast proliferationinhibition activity gradually increased, inhibition and concentration andtime of administration were positively correlated.②Ophiopogon soup ofhigh, medium and low dose group HSAS1serum inhibit the growthinhibitory effect of high-dose group the strongest possible amount ofmedicine needed to achieve a serum stock volume, in order to have a significant role.③Ophiopogon soup inhibition of skin fibroblastproliferation, suggesting that it may have a therapeutic effect on SSc.④thinChi glycopeptide HSAS1inhibition of proliferation in serum below eachmedicine, clinical medicine combination therapy may be considered in SSc.⑤promote apoptosis inhibition may be one of the mechanismsOphiopogonis soup HSAS1proliferation.⑥Ophiopogon soup to someextent, reduce human skin fibroblasts and collagen ⅠmRNA expressionlevels may alter the levels of certain cytokines in the extracellularmatrix(ECM), toregulate the metabolism of connective tissue.⑦Ophi opogonsoup SSc role of further study. |
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