The Effects Of Four Inverse Scattered Decoction On Phosphorylation Of Smad,PDGF-BB And Its Receptor In Liver Tissue Of The Immunity Hepatic Fibrosis Rats

【Objective】To observe the therapeutic effects of four inverse scattered decoction on the rats withliver fibrosis induced by pig serum. Studying the effects of four inverse scattered decoctionon Psmad2, Psmad3, PDGF-BB and its receptor in Liver Tissue of the Immunity HepaticFibrosis Rats, in order to know which blocked the signal transduction pathway.【Methods】The subject plans to study with the immunity female rats with hepatic fibrosis that wascaused by pig serum.80female Wistar rats, of healthy, and120～130g weight, wererandomly divide into normal group, pathologic model group, Four inverse scattereddecoction group, Four inverse scattered decoction flavored in the high dose group, Fourinverse scattered decoction flavored in the middle dose group, Four inverse scattereddecoction flavored with low dose group, Four inverse scattered decoction flavoredprevention group, colchicine group,10rats in each group. Using HE and Masson stainingmethod to observe changes in liver tissues of cell morphology, intragastric administrationand the expression of serological and immunohistochemical staining index in each group.【Results】The experiment was modeled after6weeks in the implementation, except for thenormal group and the prevention group, other groups were observed by HE staining.Wefound that in the liver tissue of model rats the hepatocytes had more obvious degenerationand necrosis. The results suggest that: using the model pig serum-induced liver fibrosis inrats were successful.Using HE and Masson staining method to observe the treatment has been carried outover the rats which belong to different four inverse scattered decoction dose groups.Theresults suggest that: Four inverse scattered decoction can improve the level of liver fibrosisand liver cell degeneration, necrosis, infiltration of inflammatory cells, four inverse scattered decoction can significantly reduce the degree of deposition of collagen fibers and fiber septa.For serological indicators, four inverse scattered decoction flavored in the high dose groupand prevention group can effectively reduce the total bilirubin and increase albumin, theratio of the white ball, which have the significant therapeutic functions on liver fibrosis. Theeffect of immunohistochemical staining index is remarkable:the decoction can reduce theexpression of platelet derived growth factor beta and its receptor （P＜0.05）and theexpression of phosphorylation of smad protein（P＜0.05）.【Conclusion】The research on intervention of “Four inverse scattered decoction” for anti liver fibrosisis obviously effective. Four inverse scattered decoction flavored has remarkable significancein the clinical treatment of liver fibrosis and shows significant intervention effect in anti liverfibrosis, it can provide a reliable theoretical basis and practical application value for theprevention and treatment of liver fibrosis.