The Expression And Clinical Pathological Significance Of AP-2γ And OCT-4in Lung Adenocarcinoma Tissues

Nowadays, lung cancer is the leading cause of cancer-related mortality and contributes to more than1million deaths every year worldwide. In China, the number of lung cancer patients is increasing and lung cancer has become the leading and the second largest cause of cancer-related mortality in men and women, respectively. Primary adenocarcinoma of lung has replaced squamous cell carcinoma as the commonest histological subtype of lung cancer and the incidence of primary lung adenocarcinoma appears to be rising, accounting for almost half of all lung cancers. In2011, the International Association for the Study of Lung Cancer/American Thoracic Society/European Respiratory Society (IASLC/ATS/ERS) proposed a new classification of invasive lung adenocarcinomas to provide uniform terminology and diagnostic criteria. There were several important modifications and changes to the2004World Health Organization (WHO) classification that applied to lung adenocarcinoma.In recent years, since the improvement in diagnostic technologies for lung cancer, an increasing number of patients are being diagnosed in the early stages of the disease.Despite significant improvements in radiologic imaging and multi-modality anti-cancer therapy, the5-year relative survival rate for lung cancer in the worldwide remains dismal, that is, at only16%in2007compared to12%in1975, thus indicating little improvement in survival rate over a period of3decades. Therefore, it is very necessary to identify invasion and metastasis related molecules of lung adenocarcinoma, which will be of great importance in fields such as clinical therapy, prevention and the improvement of patient’s survival rate and quality of life.Transcription factor OCT-4, which plays an important role in embryonic development, gives full play to a variety of biological functions in different developmental periods and differentiations. The effect of OCT-4is realized through the regulation of the target genes.In this study, we found OCT-4binding sites within the sequence of AP-2y promoter region. OCT-4inhibited the transcriptive activity of AP-2Y and influenced the expression of AP-2y gene in protein expression level.To elucidate the possible role of AP-2γ and OCT-4in lung adenocarcinoma, in this study, expressions of AP-2Y and OCT-4proteins were investigated by immunohistochemistry, and correlations between expressions of AP-2Y and OCT-4and clinicopathological features were analyzed. The study above will provide the theoretical basis for AP-2Y and OCT-4mediated gene target therapy of lung adenocarcinoma.Methods1.Over-expressions of AP-2Y and OCT-4protein were detected by immunohistochemistry method in85cases of lung adenocarcinoma tissues and23cases of normal lung tissues, and the relationship between their expressions and clinical pathological parameters was analyzed.2.The data from experiment was analyzed using SPSS17.0software. Differences among groups was analyzed with chi square test and the correlation between AP-2Y and OCT-4protein expression was analyzed using Spearman correlation analysis. P<0.05was considered to be statistically significant. Results1. AP-2γ protein is mainly localized in nuclear, showing pale yellow to brown granules. There is over-expression of AP-2Y protein in lung adenocarcinoma tissues, and positive ratio is67.1%(57/85), significantly higher than normal lung tissues26.1%(6/23), respectively, there are significant differences among the groups (χ2=12.502, P<0.05).2.Expression of AP-2Y is closely related gender, histological grade, lymph node metastasis, TNM staging and histological typical (χ2=6.559,21.237,7.728,9.028and12.388; P<0.05), but not related to age (χ2=0.732, P>0.05).3. OCT-4protein is mainly localized in nuclear, showing pale yellow to brown granules. There is over-expression of OCT-4protein in lung adenocarcinoma tissues, and positive ratio was44.7%(38/85), significantly higher than normal lung tissues0(0/23), respectively, there are significant differences among the groups (χ2=115.864, P <0.05).4.Expression of OCT-4is closely related histological grade, TNM staging and histological typical (χ2=19.929,8.481and17.031; P all<0.05), but not related to age, gender, lymph node metastasis(χ2=0.469,1.746and1.519, P>0.05).5. AP-2Y and OCT-4protein show positive correlativity.(P<0.05).Conclusion1. Over-expressions of AP-2Y and OCT-4protein in lung adenocarcinoma tissues are significantly higher than those in normal lung tissues, and AP-2Y at high level plays an important role in occurrence and development of lung adenocarcinoma.2. The expressions of AP-2y and OCT-4protein in lung adenocarcinoma are positively correlated, suggesting that they play a related role in occurrence and development of lung adenocarcinoma.