The Mechanism Of Targeting NF-κB Signaling Pathway To Inhibit Non-hodgin’s Lymphoma Cell Proliferation With Eriocalyxin B

Background and purposeCurrently, blood diseases are the serious threat to human health, which accountfor a large proportion of malignant lymphoma in the blood system diseases.Malignant lymphoma is broadly divided into non-Hodgkin ’s lymphoma (Non-Hodgkin’s lymphoma, NHL) and Hodgkin ’s lymphoma (Hodgkin’s lymphoma, HL)two types. Different types prognosis and survival are also different. With theimprovement of various treatments, the prognosis and disease-free survival ofmalignant lymphoma has been greatly improved, but every year there are a lot ofpeople suffering from malignant lymphoma,so continue to study of the mechanismsof malignant lymphoma and development of new anticancer drugs becomes aproblem to be solved. Recent studies have found that eriocalyxin B (Eriocalyxin B,EriB) is a structural analogue of Oridonin, it is an evergreen plant from flowerthinning hair Rabdosia (Isodon eriocalyx var. Laxiflora) in a purified enantiomerkaurane compounds. Research shows that in HL60, A549, MKN-28, HCT and CAtumor cell lines, eriocalyxin B showed strong cytotoxicity, which can significantlyinhibit the proliferation of tumor cells, but its exact mechanism of action in lymphoma is still unclear, so we must make the corresponding studies provide sometheoretical basis for future research more and more effective anticancer drugs.Materials and MethodsIn vitro, a series of B-and T-lymphoma cells were treated with EriB andobserved proliferation of lymphoma cells. While, Cell apoptosis and cell cycle wereanalyzed using flow cytometric assay. Expression of proteins related to apoptosis,cellcycle and cell signal transduction were assessed using Western blot.ResultEriB significantly inhibited lymphoma cell proliferation and induced apoptosisin association with activation of caspase(caspase-3, caspase-8and caspase-9) andcell cycle arrest with upregulation of proteins related to cell cycle,such as p21andp27. Meanwhile, nuclear factor NF-kBsignal transduction pathways were inhibited toinduce lymphoma cell apoptosis and cycle arrest in response to EriB.ConclusionEriB targets nuclear factor NF-kB signal transduction pathways to inducelymphoma cell apoptosis and cycle arrest and so to inhibit lymphoma cellproliferation.