| Background and ObjectivesColon cancer is one of the most common digestive tract malignancies. Its incidence has been increased significantly in China along with lifestyle changes in recent years. The occurrence, development and evolution of colon cancer are multi-step, complex biological processes. This process closely relate to a series of abnormal expression of cancer genes and tumor suppressor genes in which inhibit from the expression of tumor suppressor genes is the main trigger of tumor genesis. NPRL2(Nitrogen permease regulator-like2) is a newly discovered human tumor suppressor gene; researchers found that inactivation of the gene is closely relate with a variety of tumor occurrence and developnent This study selected colon cancer tissue and matched normal tissue; By means of RT-PCR, immunohistochemistry and Western blotting in order to detect NPRL-2mRNA and its protein expression levels:and to further explore the relationship between the expression levels of NPRL-2and the clinical pathological features of colon cancer,: to learn the role of NPRL-2in the occurrence and development of colon cancer.:to provide new clues regarding to the diagnosis and prognosis of colon cancer. Materials and methodsForty samples of colon cancer tissues with matched normal colon tissues was collected. The samples were frozen in liquid nitrogen,and placed in-70℃refrigerator after10min.1.The NPRL-2mRNA level in each group was detected by RT-PCR2.The NPRL-2protein level in each group was examined by Western blot and3.The NPRL-2protein level in each group was examined by immunohi stochemi stry4.Analyze the relationship between immunohistochemical results NPRL2clinical pathological factors associated with colon cancer.5.Analyse the data by exploiting the statistical software SSPS17.0. The relative expression of NPRL2mRNA and protein using the two paired samples test. NPRL2positive rate of protein compared using χ2test, test level α=0.05.ResultsThe expression level of NPRL2mRNA in colon cancer tissues (0.459±0.045) was significantly lower than normal tissues (0.656±0.041).(t=15.124P<0.001). Western blot analysis showed colon cancer and normal tissue protein expression levels were0.271±0.0150and0.588±0.051,(t=27.357P<0.001).The Positive rate of NPRL2protein in normal colon tissues and colon cancer tissues was87.50%(35/40) and32.50(13/40) by immunohistochemistry respectively. There was a significant difference between two groups (χ2=17.802P<0.001). The expression of NPRL2was not correlated with sex, age, as well as Dukes stages.(χ2-=0.631、0.684、0.664P>0.05).While it was correlated to the histology grading.The positive rate of NPRL2expression in the well and moderated differentiation group was higher than that in the poor-differentiation group (χ2=4.862P<0.05)Conclusions 1.The NPRL2mRNA and protein levels in colon cancer levels were significantly decreased compared with normal colon tissue.2.1n the analysis of the relationship between protein expression and clinic pathological features based on immunohistochemical results which found the expression in different degrees of differentiation NPRL2colon cancer is different. To be more specific,the protein expression levels in high and moderately differentiated colon cancer were significantly higher than the levels in the poorly differentiated colon cancer.3.These findings suggest that NPRL2inhibited during the occurrence of colon cancer, and it may be involved in regulating the differentiation of colon cancer cells as well. |
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