| ObjectiveIn this article, by observe the expression of Interleukin-21,(Signal Transducer andActivator of Transcription3in lung tissue of rat and chronic asthmaic rat, and to observethe expression changes after use BCG-Polysaccharide and Nucleic Acid injection changes,to provide the basis for the mechanism to further clarify the BCG polysaccharide nucleicacid treatment of asthma in children.Methods1.40male SD rats were randomly assigned to four groups which were clean and healthy, about a month old, weighing150-170g.the four groups were normal control group (Control group), asthma model group (Model group), Dexamethasone intervention group (DXM group), BCG-PSN group), each10.2.the experimental rats in Model group, DXM group, BCG-PSN group were intraperitoneal injected by10%egg albumin (Ovalbumin, OVA) solution of1ml contains100mg of aluminum hydroxide on the first day,the15th days. Then were atomized inhalated with2%ovalbumin solution4ml on the22th day,20minutes eachtime, once two days, lasting for12weeks. The Control group used0.9%sodiumchloride solution instead of ovalbumin in intraperitoneal injection and inhalation.3.From the first day of experiment, in Dexamethasone group,0.5h before aerosolizedOVA, each rat was given0.3mg/kg dexamethasone by means of intraperitoneal injection;In BCG-Polysaccharide and Nucleic Acid injection group, each rat was given0.45mg/kgBCG-Polysaccharide and Nucleic Acid injection by means of intraperitoneal injection.three times for every weeks, lasting twelve weeks.4. On the12th weeks of experiment, four groups of rats were anesthetized by intraperitoneal injection of chloral hydrate,then detected the airway hyper responsiveness.After test the rats were sacrificed with abdominal aorta bleeding methods,everylung tissue was detected by quantitative real-time PCR,to observer the expression of STAT3mRNA and IL-21mRNA in lung tissue, the expression of STAT3protein was detected by Western Blot. At the same time the morphology of lung tissueof rats in each group were observed.Result1.Construction of subacute asthma rat model: From the twenty-two days of theexperiment these following symptoms begin to appear:facial scratching, respiratoryfrequency speed, nodding breathing, facial cyanosis, incontinence.on the twelves weeks the airway hyper responsiveness were detected, The results suggest that airway resistance in asthma model group rats increased significantly, compared with the normal control group were statistically significant (P <0.05);HE staining of lung tissuesections suggest that bronchial and alveolar lung tissue of rats with inflammatorycells increased significantly, eosinophils increased,more viscous secretions, bronchialwall thickening, which showed that the rat model of chronic asthma was established successfully.2.after BCG-PSN intervention in lung tissue of rats with pathology: determination andairway resistance in asthma rats with intervention of BCG-PSN pathological changes, canstill be seen in BCG-PSN group compared with Model group, inflammatory reaction,inflammation; compared with DXM, the difference was not significant; however,compared with the Control group still visible inflammatory reaction. Four groups of ratswere stimulated with different concentrations of histamine hydrochloride, airwayresistance in BCG-PSN group compared with Model group, the airway resistancedecreased, there was statistically significant (P <0.05); compared with Control group, theairway resistance is increased, with statistical significance (P <0.05); compared with DXMgroup, no statistical significance.3.Changes of the expression of STAT3in lung tissue and IL-213after BCG-PSNintervention: BCG-PSN dry lung tissue of asthmatic rats, the prognosis of STAT3IL-21mRNA transcription was lower than in group Model, with statistical significance (P <0.05);compared with Control group, but significantly increased, with statistical significance (P <0.05); and DXM is compared, no significant significance. BCG-PSN intervention group the expression of STAT3is significantly lower than those of Model group, there wasstatistically significant (P <0.05); compared with Control group, but significantlyincreased, with statistical significance (P <0.05); compared with DXM, no statisticalsignificance.Conclusion1.Sensitized by OVA, excitation, to construct model of chronic asthmatic rats. STAT3,high expression of IL-21in lung tissue of rats, involved in asthma pathogenesis.2.BCG-PSN can reduce the airway hyperresponsiveness of asthmatic rats, reduceairway inflammation.3.BCG-PSN can inhibit asthma IL-21protein STAT3, expression in rats, suggestingthat the effects of BCG-PSN associated with the downregulation of expression of STAT3,IL-21in lung tissue. |
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