The Effects Of FK506(Tacrolimus) On The Structure Of Mouce Intestinal

Objective To investigate the effects of FK506(Tacrolimus) on the structure of mouce intestinal mucosa.Methods C57BL/6male mice used as the animal models were randomly divided into experimental group and control group. Experimental groups include high dose group (FK5065mg/kg gavage), medium dose group (FK5063mg/kg gavage) and low dose group (FK506lmg/kg gavage). And the mice in control group recieved the same amount saline.Method:C57BL/6male mice used as the animal models were randomly divided into experimental group and control group. Experimental groups include high dose group (FK5065mg/kg gavage), medium dose group (FK5063mg/kg gavage) and low dose group (FK506lmg/kg gavage). And the mice in control group recieved the same amount saline.In the first week, FK506was administrated once a day, and after that it was given every other day. Thirty days later, weight of mice and plasma concentration of FK506were measured. Structure and ultramicro-structure of mouse intestinal mucosa were observed by HE dying and transmission electron microscope (TEM) respectively.Results The weight of mice in experimental group is lower than that in the control group, and the higher dose of drug the mice is given, the lighter the weight of them is. The plasma concentration of FK506of high dose group is the highest, medium dose group comes second and low dose group is the lowest. Results of HE dying and transmission electron microscope (TEM) both show the damage to intestinal mucosal barrier in the experimental group, and extent of damage is positive correlated with dosage of drug.Conclusions FK506causes damage to the structure of intestinal mucosa. Its mechanism lies in the damage of intestinal mucosa cell tight link and other ultramicro-structures. Moreover, the extent of damage is directly related to the dosage of drug.