Effects Of Low Oxygen On The Express Of Related Cytokine In Human Umbilical Cord Mesenchymal Stem Cells

Objective To observe the effects of low oxygen on the express of VEGF, HGF, bFGF, SDF-1, SCF, IGF, G-CSF in human umbilical cord mesenchymal stem cells(hUCMSCs).Method The third generation of hUCMSCs were divided into hypoxia group and normoxia group, and were cultured in20%oxygen and5%oxygen, respectively. The concentrations of HGF, IGF-1, SDF-1, VEGF, bFGF, SCF and G-CSF were detected by ELLSA after culturing8h,24h,32h,48h,56h and72h, respectively.Results The concentrations of HGF, SDF-1, VEGF, SCF and G-CSF in hypoxic group was significantly higher compared to those of normoxia group after72h culturing (P<0.05); The concentration of VEGF and bFGF in hypoxic group was significantly higher than that of normoxia group after56h and32h culturing, respectively (P<0.05). However, the concentration of IGF-1in hypoxic group had no significant changes compared to that of normoxia group (P>0.05)Conclusions The continuous express of cytokines in hUCMSCs, such as HGF, IGF-1, SDF-1, VEGF, bFGF, SCF and G-CSF, could be found, and the express of the above cytokines except for IGF-1could be increased by5%oxygen.