Influences Of EPO On Brain Tissues Of Premature Rats With Intrauterine Infection WMD Including CD68, GFAP Level And Neuroethology

Objective:To Study the neuroprotective effect of rhEPO on premature rats with intrauterine infection WMD through detection of the changes in CD68and GFAP in brain tissue and assessment of neuroethology.Method:Selected20wistar rats that had been pregnant for15d and divided them into infection group (n=15) and control group (n=5) by ratio3:1at random, conducted intraperitoneal injection of LPS (0.3mg/Kg) and equivalent stroke-physiological saline solution on15th day’s pregnancy of Wistar rats in both groups respectively and conducted cesarean section to take out fetal rats on21th day’s pregnancy; observed pathological changes by taking out fetal rats (n=10) and new rat brain tissue (n=10) hematoxylin eosin (HE) staining in two groups respectively; randomly selected40newborn rats in the control group as the premature birth control group; selected80newborn rats in the infection group and randomly divided into EPO treatment group (n=40) and infection control group (n=40). Regarding EPO treatment group, conducted intraperitoneal injection of rhEPO (5000IU/Kg) promptly after the birth. Regarding premature control group and infection control group, conducted intraperitoneal injection of equivalent saline solution simultaneously. As for newborn rats in three groups, took10premature rats on lrd,3rd and7th day of their birth respectively and took out brain by perfusing formaldehyde; detected the level of CD68and GFAP in tissue through immunofluorescent staining; as for these three groups, took10premature rats on30th day after their birth respectively to observe neuroethology through open field test, suspension test, ramp test and resistance to capture reaction.Result:1. The placenta of pregnant rats in the infection group featured congestion of blood vessel and edema with a lot of visible neutrophile granulocytes infiltrated. Pale HE staining and loose structure of newborn rats brain tissue in the infection group; the thickness of nerve fiber was uneven with disorder in move. It could be seen that there were deeply stained and few protuberant less-differentiated nerve cells. The number of glial cells was increased. It could be seen that there were changes in apoptosis such as karyopyknosis, loose cytoplasm and cell shrinkage.2. Each group had no positive staining of CD68and GFAP at1d;in infection control group and EPO treatment group, CD68and GFAP level in brain tissue of rats of3d and7d old was remarkably increased compared to that in the premature control group.There was statistical significance in difference among each (P<0.05); CD68and GFAP level in brain tissue of rats of3d and7d old in EPO treatment group was remarkably reduced compared to that in the infection control group. there was statistical significance in difference (P<0.05). CD68and GFAP level in brain tissue of rats of7d old in EPO treatment group was remarkably reduced while that of3d old in the infection control group was increased. There was statistical significance between the both (CD68and GFAP level in brain tissue of rats).3. Neurobehavior test:neurobehavioral score of rats of30d old in the infection control group was remarkably lower than that in the premature control group. The neurobehavioral score of premature rats in EPO treatment group was remarkably higher than that in the infection control group. There was statistical significance in difference (P <0.05).Conclusion:1. By intraperitoneal injection of LPS into pregnant rats, the premature WMD rat model could be built successfully.2. The inflammatory reaction and reactive hyperplasia of WMD of newborn premature rat caused by intrauterine infection could be mitigated through application of rhEPO into newborn premature rat at the early stage. It has certain therapeutic effect on WMD of newborn premature rats with intrauterine infection.3. Neurogenesis and cognitive function of premature rats with WMD caused by intrauterine infection could be ameliorated by applying rhEPO in the early stage.