Research Of Adipose Derived Stem Cell For Repairing Chronic Ultraviolet Radiation Damage Of The Skin

Objective:This study draws assistance from chronic ultraviolet radiation damage model of ratsto investigate transplantation of ADSCs which maybe provide a choice of clinicaltreatment for skin photoaging.1. Study growth and differentiation of human ADSCs byextraction, cultivation and identification.2. Improved the methods of establishingchronic ultraviolet radiation damage model.3. To explore the feasibility of treatment ofskin photoaging by ADSCs transplantation, investigate new ideas for the clinicaltreatment of photoaging.Methods:Part I: Adipose tissues donated by liposuction of patients. Extraction of ADSCs bythe method of enzyme digestion, then seeded in culture dishes, used the HG-DMEMculture medium. After the cells adherent, used the inverted microscope to observe theprimary cells. Culturing to the third generation identified the cells by flow cytometry.And then, we induced the cells into adipocyte and osteocyte by different inducers.Part II: Established chronic ultraviolet radiation damage model of rats’ dorsal skin:remove back hair of rats, through daily exposure to UV, and ultimately causing aging.Cutting aging skin to observe by H&E and VVG staining through inverted microscope.Part III: Use the third generation stem cells suspension adjusted the concentration to1.5x105/ml. Each rat’s back marked3regions (called a, b, c), injected evenly ADSCssuspension with each regional1ml dose to the treatment group. Uniform with the samedose of PBS injected to control group and blank group. Injection cells once a week forfour weeks. After the end of treatment, we harvested skin tissues three times, in three weeks. We observed cells by H&E and VVG staining under inverted microscope. Wetested activities of SOD and the content of MDA. Explore ADSCs’ therapeutic effects ofchronic exposure to UV damage.Results:Part I: Primary cells are spindle-shaped, irregular triangles or polygons, growth as abundle or whirlpool. After passage, they proliferation faster, and usually reach90%fusion in a week. It highly consistent with the phenotype of mesenchymal stem cellsexpress. Continually culture, ADSCs showed good adipogenic and osteogenic ability.Part II: using the most simple and General naked eye observation, H&E stained andVVG staining tissue sections were determined that the model was successfullyestablished.Part III: After transplantation, chronic ultraviolet radiation injured skin has somechange, such as dermal collagen synthesis increased, thickness of the dermis increased,thickness of epidermis reduced, inflammatory cell infiltration improved, SOD activityincreased, MDA peroxide deposition reduced.Conclusion:Part I: We have a deeper understanding of ADSCs by using the ADSCs extraction byenzymatic digestion of the classic method, and identify them in accordance withinternational standards. Through the growth curve, we learned the growth characteristicsand have a more intuitive understanding of morphological features. These have laid thefoundation for the follow-up experiment.Part II: Improved the establishing of chronic ultraviolet radiation damage model,make it more efficient.Part III: experimental results confirm that ADSCs can reverse chronic UV damageto treatment the skin aging.