The Study Of The Changes Of Serum Angiotensin-(1-7) In Patients Of Congenital Heart Disease With Pulmonary Arterial Hypertension

Objective:We observed the Angiotensin-(1-7)(Ang-(1-7)) protein levels in the serum with Congenital Heart Disease (CHD) combined with pulmonary arterial hypertension (PAH), and investigated the correlation of those with pulmonary arterial hypertension.Methods:90patients with Congenital Heart Disease and30normal control patients were collected,The patients with congenital heart disease were divided into27cases of non-pulmonary arterial hypertension group,42cases of mild to moderate pulmonary arterial hypertension, and21cases of severe pulmonary arterial hypertension.The serum level of Ang-(1-7) protein levels were detected by enzyme-linked immunosorbent assay(ELISA), Statistical analysis was performed by using SPSS17.0software.Results:1. The Ang-(1-7) protein levels in plasma measured by enzyme-linked immunosorbent assay were (17.39±1.33)pg/ml,(26.23±1.21)pg/ml,(18.84±1.59) pg/ml,(12.52±1.85) pg/ml separately in normal control group(group A), Congenital Heart Disease with non-pulmonary arterial hypertension group(group B), Congenital Heart Disease with mild to moderate pulmonary arterial hypertension group(group C) and Congenital Heart Disease with severe pulmonary arterial hypertension group(group D) before surgery. The levels of Ang-(1-7) protein levels showed significantly difference among different groups (P<0.05).2. Correlation and regression analysis showed a negative correlation between Pulmonary arterial pressure and Ang-(l-7) protein levels(r=-0.841, p<0.001). 3. The Ang-(1-7) protein levels in plasma measured by enzyme-linked immunosorbent assay were (17.39±1.33) pg/ml,(21.73±3.91) pg/ml,(12.52±1.85) pg/ml in normal control group, Congenital Heart Disease with non-cardiac remodeling group, Congenital Heart Disease with cardiac remodeling group.The levels of Ang-(1-7) protein levels showed significantly difference among the three groups (P<0.05).Conclusions:1. Ang-(1-7) protein level in the patients with Congenital Heart Disease combined with non-pulmonary arterial hypertension have significantly increased. However, Ang-(1-7) protein levels significantly decreased following pulmonary arterial pressure increased. There was a negative correlation between Pulmonary arterial pressure and Ang-(1-7) protein level.2. Ang-(1-7) protein levels in the patients with Congenital Heart Disease combined with cardiac remodeling have significantly decreased.3. Ang-(1-7) protein levels can be the predictive index of patients with Congenital Heart Disease combined with pulmonary arterial hypertension. When Ang-(1-7) protein levels lower than16.47pg/ml,it shows that the patients with Congenital Heart Disease may have pulmonary arterial hypertension.