| Objective:To establish MA dependent rat module, then observe the changes of ChAT and AchE after the application of Nucleus Accumbens Electrical Stimulation onto rats, and discuss the influences from the radio frequency electrical stimulation on the nucleus accumbens metabolism changes of the MA dependent rats. Methods:divide160SD rats into contrast groups randomly, and establish rat models, according to the dependent time, into groups of one-week, two-week, three-week and eight-week. Each dependent group can be further divided into3subgroups respectively:MA dependent group, MA dependent+sham-operation group, MA dependent+operation groups. Firstly utilize brain solid positioner to locate nucleus accumbens, then perform craniotomy by drill, and insert needle electrodes. After that, adopt radio frequency electrical stimulator to apply radio frequency electrical stimulation (1mA,30s) to the respective rats’nucleus accumbens. Then after three days’ recovery of stimulation operation, inject MA for seven days. Then utilize immunohistochemistry, situhybridization, western blotting and real time quantitative PCR techniques to inspect the changes of AchE and mRNA. Results:1. The establishment of methamphetamine succeeds;2.Compared with the MA dependent group, the time spent on drug-pair compartment of CPP experiment of one-week and two-week groups are extended, and there are obvious stereotyped behaviors(p<0.05); there are no outstanding differences in the stereotyped behaviors scoring for four-week and eight-week groups (p>0.05).3. Compared with that of the solely dependent group, there is obvious difference in stereotyped behavior score of rats from one-week and two-week electrical stimulation groups (P<0.05); there is no significant change in four-week and eight-week groups(P>0.05);7days after the re-administration, stereotyped behavior score of rats from one-week and two-week electrical stimulation groups is significantly different from that of rats from solely dependent group (P <0.05), and there is no significant difference in rats from four-week and eight-week groups (P>0.05);4. Comparing the different subgroups of the four experimental groups with the blank control group, AchE and its mRNA expression of one-week and two-week groups increase in MA dependent group and MA dependent+sham-operation group. However, when it comes to MA dependence+operation group, it has dropped significantly (p<0.05). The changes of AchE and its mRNA expression in four-week and eight-week dependent groups are not outstanding;5.ACHE and its mRNA expression of MA dependent one-week group have dropped (p<0.05); there are no outstanding differences in MA dependent+operation one-week、two-week、four-week、eight-week groups compared with MA dependent group Conclusion:1. The model of MA dependence rat is established by intraperitoneal injection of many times and conditioned place reference.2. Functions of nucleus accumbens cholinergic neurons of MA short period dependent rats are suppressed,while the suppression of nucleus accumbens cholinergic neurons of MA long period dependent rats are eliminated, which is probably related with drug resistance.3. The radio frequency electrical stimulation operation has obviously activated nucleus accumbens cholinergic neurons systems, removed the suppression status of cholinergic neurons brought by MA dependence and has obviously relieved the dependent effects of MA short-period dependent rats. But it does not have obvious effects for MA long-period dependent rats. |
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