Experiment Research On HCV Infection Of Infantile Tree Shrews

Hepatitis C virus (HCV) is a small enveloped virus that causes chronic hepatitis worldwide. HCV belongs to the genus Hepacivirus of the family Flaviviridae. Its genome comprises9.6kb of single-stranded RNA of positive polarity.The virus is40-50nm in diameter and the main replication in liver cells. Humans are the natural host of HCV, HCV chimpanzees are sensitive animals. The hepatitis C virus can cause chronic hepatitis, fatty liver, cirrhosis and even liver cancer.Tree shrew is a live only in Southeast Asia, a non-primate mammals. It can infect many endogenous viruses, such as hepatitis B virus HBV, also showed susceptibility of HCV. Many literature indicates that tree shrews which infected with HCV although unlike humans and chimpanzees as a continuing high titer viremia, but the approximately1/3of tree shrews show the emergence of a transient infection, in addition to in vivo infection with the virus as a tree shrew try animal models, as well as in vitro infection model in tree shrews liver cell-based. The evidence strongly description tree shrews have become HCV infection in animal models. The use of tree shrews as an animal model for HCV infection has not yet been attempted. This article study on part of the characteristics of tree shrews infected by HCVIn this paper, using the hepatic portal vein injection method, injection of1mL to the liver of5-week-old young tree shrews load of HCV viral supernatant1.3×107copies/mL J6/JFH-1type, and after the third day, each pumping0.6mL blood of non-anticoagulant and anti-clotting50ul blood of three consecutive months,50ul anticoagulant for blood test. The positive band was detected through nested PCR.The similarity of recycling the positive band and sequencing was99%after compared with HCV J6/JFH1-QC69(NS4A)(KF693783.1) by BLAST on the GenBank. Make sure the positive band was the HCV virus fragment. The positive ratio was70.0%on the third day, the positive ratio was100%on the fifth week, the positive ratio was88.9%on the sixth week. Using Taqman probe Real-Time PCR method for RNA quantitation find the third and fifth weeks after vaccination got a viral load which were6.07×103copies/mL and2.04x103copies/mL,respectively.Histopathological examination revealed some seen focal lymphocytic infiltration in Histology portal area of tree shrew. The result of immunohistological staining support the fact that the HCV antigen expression in the infected tupaia is exist. These results indicate infantile tree shrews have a prevalence of HCV-positive. Some young tree shrews infected with HCV can become a chornic infection animal model.