Proteomics Analysis Of Pleural Effusion From Lung Cancer And Tuberculosis Patients

Objective:To describe the profile of the proteins in pleural effusion in lung cancer and tuberculosispatients, and to find the biomarkers protein for distinguish lung cancer and tuberculosis,Nano-HPLC-ESI-QTOF-MS/MS was used to identify the proteins in pleural effusion from suchpatients.Background:Lung cancer and tuberculosis are the most common disease in china. These two diseases arethe most common cause for pleural effusion. Tuberculosis pleural effusion and lung cancer pleuraleffusion are different with pathogenesis and therapy. But they have the similar presence in thephysical and chemical character. As a result so many pleural effusion patients are misdiagnosedclinically. It is valuable for finding the biomarker to distinguish tuberculosis and lung cancerpleural effusion.Method:1. Pleural effusion samples were collected from the lung cancer and tuberculosis patients.2.The proteins in the samples were separated by1D-SDS-PAGE.3. The gel was cut depending on theprotein bands distribution, and digested by the trypsin.4. The proteins on the gel were identified bythe Nano-HPLC-ESI-QTOF-MS/MS.5. The characters of proteins from the lung cancer andtuberculosis were got by the enrichment analysis separately.6. The differential proteins as thepotential biomarkers were got by comparing the database of lung cancer and tuberculosis pleuraleffusion.Result:1.The individual difference of the lung cancer samples was more obvious than tuberculosis onthe1DE-SDS-PAGE.2. The proteins participating the blood coagulation and inflammatoryresponse were enriched in both group. The proteins participating the cell cycle and cell motionwere enriched in tuberculosis group. The proteins participating the Ras signal pathway and the methylation were enriched in the lung cancer group.3. The Mycobacterium tuberculosis proteinMMPL1and Rv2567were identified in the most of the tuberculosis sample but not in every lungcancer sample. Seven human proteins including APOL1, C1qb, FCN3, HPT, HPTR, IL-1α,prothrombin were differently expressed in the two groups.Conclusion:1. Lung cancer pleural effusion has more heterogeneity than tuberculosis on the proteinsmolecular weight distribution.2. The proteome characters of lung cancer and tuberculosis pleuraleffusion were difference in cell cycle, signal pathway etc.3.2Mycobacterium tuberculosis proteinsand7human proteins were potential biomarker to distinguish lung cancer and tuberculosis pleuraleffusion.